Alleles of the Mads-box domain used to control palm shell phenotypes
A technology of allele and shell phenotype, applied in genetic engineering, plant genetic improvement, application, etc.
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[0150] Example 1. Recognition of alleles 3-11, corresponding to nucleotide sequences SEQ ID NOs: 12-21 and polypeptides SEQ ID NOs: 3-11
[0151] We previously reported homozygous (eg AVROS / AVROS, MPOB / MPOB or AVROS / MPOB) and tenera oil palm fruit phenotypes, heterozygotes with one wild-type dura allele (eg AVROS / dura or MPOB / dura), AVROS and MPOB mutations in exon 1 of the SHELL gene are responsible for the pisifera oil palm fruit phenotype. While tenera oil palm is the preferred phenotype for commercial oil palm production, it is difficult to completely prevent the emergence of wild-type dura palm in commercial populations. To assess the extent of dura contamination within commercial oil palm populations and to search for new alleles of the SHELL gene that produce the pisifera / tenera AVROS and sh MPOB ) tested 5,158 oil palm trees from 6 different smallholders in Malaysia for the presence of AVROS and / or MPOB alleles using an allele-specific PCR assay. As expected, most p...
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