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Method for extracting cyanidenon compounds and cynaropicrin

A technology of luteolin and cynopicrin, which is applied in chemical instruments and methods, organic chemistry methods, sugar derivatives, etc., can solve the problems of difficulty in deep development and utilization of artichoke, and limited research on separation and purification of effective components. The effect of high extraction purity and simple extraction method

Active Publication Date: 2019-02-12
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing technology has limited research on the separation and purification of its active ingredients, which makes it difficult to carry out the deep development and utilization of artichoke

Method used

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  • Method for extracting cyanidenon compounds and cynaropicrin
  • Method for extracting cyanidenon compounds and cynaropicrin
  • Method for extracting cyanidenon compounds and cynaropicrin

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Dry the artichoke and pulverize it with a pulverizer. At room temperature, add the artichoke powder into a 70% ethanol aqueous solution with a volume fraction of 1:10 for leaching for 72 hours. Stir constantly during the leaching, and then The extract is first vacuum filtered with a filter cloth, and then vacuum filtered with a filter paper, and the filtrate is concentrated under reduced pressure to obtain a crude extract. The solid content of the crude extract is 0.1 g / ml.

[0051]Soak the AB-8 macroporous resin in absolute ethanol for 24 hours, and keep stirring during the soaking. Afterwards, put the resin particles and absolute ethanol into a column (the column volume is 1 L), and wash with absolute ethanol until the effluent is not turbid after adding water (1:5), and then rinse with pure water until there is no alcohol smell. Put the pure water in the column until it is equal to the resin, slowly add 1L of the crude extract to the chromatography column, until the...

Embodiment 2

[0059] Dry the artichoke and pulverize it with a pulverizer. At room temperature, add the artichoke powder into a 70% ethanol aqueous solution with a volume fraction of 1:8 for extraction for 70 hours. Stir continuously during the extraction, and then The extract is first vacuum filtered with a filter cloth, and then vacuum filtered with a filter paper, and the filtrate is concentrated under reduced pressure to obtain a crude extract.

[0060] Soak the AB-8 macroporous resin in absolute ethanol for 20 hours, and keep stirring during the soaking. Afterwards, put the resin particles and absolute ethanol into a column (the column volume is 1 L), and wash with absolute ethanol until the effluent is not turbid after adding water (1:5), and then rinse with pure water until there is no alcohol smell. Put the pure water in the column until it is equal to the resin, slowly add 1L of the crude extract to the chromatography column, until the resin in the lower layer of the column is all ...

Embodiment 3

[0065] Dry the artichoke and pulverize it with a pulverizer. At room temperature, add the artichoke powder into an aqueous ethanol solution with a volume fraction of 70% for 71 hours at a material-to-liquid ratio of 1:9. Stir continuously during the extraction, and then The extract is first vacuum filtered with a filter cloth, and then vacuum filtered with a filter paper, and the filtrate is concentrated under reduced pressure to obtain a crude extract.

[0066] Soak the AB-8 macroporous resin in absolute ethanol for 22 hours, and keep stirring during the soaking. Afterwards, put the resin particles and absolute ethanol into a column (the column volume is 1 L), and wash with absolute ethanol until the effluent is not turbid after adding water (1:5), and then rinse with pure water until there is no alcohol smell. Put the pure water in the column until it is equal to the resin, slowly add 1L of the crude extract to the chromatography column, until the resin in the lower layer of...

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Abstract

The invention provides a method for extracting cyanidenon compounds and cynaropicrin. The method comprises the following steps of crushing artichoke; then, performing leaching to obtain a coarse extraction solution; feeding a sample to an AB-8 type macroporous resin chromatographic column; collecting eluant; performing concentration freeze drying to obtain a first coarse extract and a second coarse extract; dissolving the first coarse extract by a methanol water solution; feeding the sample of the first coarse extract to an RP-C18 chromatographic column; collecting eluant; performing concentration freeze drying to obtain a first pre-separated article and a second pre-separated article; dissolving the first pre-separated article by an acetonitrile water solution; performing first liquid phase semi-preparation; collecting a fluid section; performing concentration freeze drying to obtain a first target compound and a second target compound; performing recrystallization on the second coarse extracts and the second pre-separated material; performing dissolution by 50-percent methanol; performing second liquid phase half preparation; collecting the fluid section; performing concentrationfreeze drying to obtain a third target compound. The method provided by the invention has the advantages that the target compounds are effectively separated from the artichoke; the extraction purityis high; the extraction method is simple; the method is favorable for the deep development of the artichoke.

Description

technical field [0001] The invention relates to the field of extraction and purification, in particular to a method for extracting luteolin compounds and styrenin. Background technique [0002] Artichoke is a perennial herb of the family Asteraceae, also known as artichoke, artichoke, chrysanthemum thistle, lotus lily, French lily, and its scientific name is Cynara scolymus L. Native to the Mediterranean coast, southern Europe and North Africa, it was introduced to Shanghai, China by France in the 19th century, and is now mainly distributed in Shanghai, Zhejiang, Yunnan, Hunan, Shandong and Beijing. Artichoke has a high edible value and has the reputation of "the king of vegetables". [0003] Artichoke leaf extract has been used in folk medicine for a long time. Its in vitro and clinical tests have shown that artichoke extract has antioxidant, antibacterial, anti-cancer and anti-tumor properties, liver protection, hypoglycemia, improvement of digestion and improvement of hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08C07D307/93
CPCC07B2200/07C07D307/93C07H1/08C07H17/07
Inventor 王元清曹清明李群王蔚婕李安平
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY