Unlock instant, AI-driven research and patent intelligence for your innovation.

Kit for synchronously detecting paddy rice grain width genes GW5 and GW8 and multiple PCR (Polymerase Chain Reaction) detection method

A technology of simultaneous detection and detection method, applied in the field of agricultural biology

Inactive Publication Date: 2019-02-12
HENAN AGRICULTURAL UNIVERSITY
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method to simultaneously screen and identify two grain width genes, GW5 and GW8

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for synchronously detecting paddy rice grain width genes GW5 and GW8 and multiple PCR (Polymerase Chain Reaction) detection method
  • Kit for synchronously detecting paddy rice grain width genes GW5 and GW8 and multiple PCR (Polymerase Chain Reaction) detection method
  • Kit for synchronously detecting paddy rice grain width genes GW5 and GW8 and multiple PCR (Polymerase Chain Reaction) detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0038] Unless otherwise specified, the technical means used in the following examples are well known to those skilled in the art, and the instruments, kits, and rice materials used are all commercially available or common varieties for breeding.

[0039] Example of a kit for simultaneous detection of rice grain width genes GW5 and GW8

[0040] The kit for synchronous detection of rice grain width genes GW5 and GW8 of this embodiment includes specific primers for the GW5 gene and specific primers for the GW8 gene;

[0041] The specific primers for the GW5 gene are:

[0042] GW5-F: 5'-GTCGGTCGTTGGAGGCA-3',

[0043] GW5-R: 5'-GGGACTATGTGGGATAGGAT-3';

[0044] The specific primers for the GW8 gene are:

[0045] GW8-F: 5'-AAAGAGACAGCCACGGAATC-3',

[0046] GW8-R: 5'-ATCTTGAGATCCCACTCCAT-3'.

[0047] The position of the GW5 gene deletion frag...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for synchronously detecting paddy rice grain width genes GW5 and GW8 and a multiple PCR (Polymerase Chain Reaction) detection method, and belongs to the technical fieldof agricultural biology. The kit for synchronously detecting the paddy rice grain width genes GW5 and GW8 comprises a GW5 gene specific primer, a GW8 gene specific primer, a 2*Taq Master mix and a DNAmarker. By utilizing the kit and the multiple PCR detection method of the invention, by optimizing a PCR reaction system and program, the multiple PCR method capable of synchronously detecting the GW5 and GW8 genes is established, and two target genes can be detected by virtue of one-step PCR reaction. The method is simple in operation procedures, low in cost, short in detection time and capableof improving the detection efficiency and providing evidence for molecular marker assisted breeding and variety improvement of paddy rice varieties with high quality and high yield.

Description

technical field [0001] The invention relates to a kit for synchronously detecting rice grain width genes GW5 and GW8 and a multiplex PCR detection method, belonging to the field of agricultural biotechnology. Background technique [0002] Rice yield is composed of the number of panicles per plant, the number of grains per panicle, and the thousand-grain weight. Among them, the thousand-grain weight is an important grain trait, which is affected by the grain shape, and the grain shape also directly affects the quality of rice. Rice grain shape is a three-dimensional structure composed of grain length, grain width and aspect ratio, which is a complex agronomic trait. Rice grain width is a quantitative trait controlled by different genetic systems such as embryo, endosperm and maternal plant. By optimizing the grain width gene, the yield and quality of rice can be greatly improved. [0003] The GW5 gene is a main effect of controlling rice grain width. Some studies have locat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/16C12Q2537/143
Inventor 李俊周刘李鑫哲赵全志杜彦修孙红正彭廷张静
Owner HENAN AGRICULTURAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com