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Thin-layer identification method for fat-soluble components in radix pseudostellariae

A technology for thin-layer identification and ginseng medicinal materials, which is applied in the field of thin-layer identification of fat-soluble components in Taizi ginseng medicinal materials

Active Publication Date: 2019-02-19
福建省中医药科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, there are certain limitations in evaluating the quality of Radix Pseudostellaria Pseudostellariae only based on the characteristics of amino acid TLC.

Method used

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  • Thin-layer identification method for fat-soluble components in radix pseudostellariae
  • Thin-layer identification method for fat-soluble components in radix pseudostellariae

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Embodiment 1

[0021] A method for TLC identification of fat-soluble components in Pseudostellaria Radix Pseudostellariae, comprising the following steps:

[0022] Preparation of the test solution: take 5g of Radix Pseudostellaria powder produced in Anhui, Fujian (Zheshen No. 2), Guizhou, Jiangsu, Shandong, and Fujian (Zheshen No. 3), add petroleum ether (60-90°C) 150ml, Sonicate for 30 minutes, filter, concentrate the filtrate to dryness, add 2 ml of petroleum ether (60-90°C) and transfer to a 10 ml round bottom flask, concentrate to dryness, add 2 ml of 0.5 mol / L potassium hydroxide-methanol solution, and bathe at 60°C 30 min, let it cool, add 2 ml of 14wt% boron trifluoride-methanol solution, bathe in 60°C water for 30 min, let it cool, add 2 ml of n-hexane accurately, add 2 ml of saturated sodium chloride solution, shake well, take the upper layer to get different Pseudostellaria heterophylla test solution.

[0023] Preparation of reference substance solution: 5g of Pseudostellariae ref...

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Abstract

The invention provides a thin-layer identification method for fat-soluble components in radix pseudostellariae. The method uses a petroleum ether-chloroform-ethyl acetate solution having a volume ratio of 10:2:1 as a developing solvent. The various fat-soluble components in radix pseudostellariae are more effectively separated under the optimized thin-layer chromatographic analysis conditions, andthe mixed pseudo-products such as ophiopogon japonicus, stemona japonica, and lophatherum gracile are well distinguished. The method expands the quality evaluation index of the radix pseudostellariaeand enriches the content of the quality evaluation.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and in particular relates to a method for thin-layer identification of fat-soluble components in the medicinal material of Radix Pseudostellariae. Background technique [0002] Pseudostellariae Pseudostellariae Pseudostellaria heterophylla (Miq.) Dried root tuber of Pax ex Paxet Hoffm. For my country's traditional Chinese medicine [National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China. 2015 edition, one part. Beijing: China Medical Science and Technology Press, 2015: 68. ]. Sweet, slightly bitter, flat. Return spleen, lung meridian. It has the effects of replenishing qi and invigorating the spleen, promoting body fluid and moistening the lungs. For deficiency of the spleen and fatigue, loss of appetite, weakness after illness, deficiency of Qi and Yin, spontaneous sweating and thirst, dryness of lung and dry co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 阚永军胡娟蒋畅庞文生杨晗
Owner 福建省中医药科学院