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Method for quick qualitative detection of existing form of boron element in food

A qualitative detection and detection method technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of non-traceability, loss of enterprises, inability to distinguish boron compounds, etc., to avoid false positive results and promote separation and purification.

Inactive Publication Date: 2019-02-22
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that these methods only target the boric acid produced after digestion, and cannot distinguish different forms of boron compounds before digestion, and cannot trace whether the detected boric acid is endogenous boron contained in the food itself or illegally added exogenous boron
This may cause false positive results in some food testing and bring unnecessary losses to related enterprises

Method used

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  • Method for quick qualitative detection of existing form of boron element in food
  • Method for quick qualitative detection of existing form of boron element in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Taking the commercially available health product calcium fructose borate as an example, TLC analysis of boron was carried out.

[0037] A kind of rapid detection method of the boron element in detecting fructose calcium borate:

[0038] (1) Dissolve a small amount of calcium fructose borate in water as the sample to be tested;

[0039] (2) Prepare dilute sulfuric acid with a concentration of 2mol / L; weigh curcumin, dissolve it in distilled water, and the concentration of curcumin is 1mg / mL; slowly add the prepared dilute sulfuric acid into the curcumin aqueous solution, according to the volume ratio of 95 :1 mix, shake well;

[0040] (3) Prepare developer (ethyl acetate: absolute ethanol = 19:1);

[0041] (4) Spotting on a TLC plate, unfolding, and developing the color;

[0042] (5) Calculate R f value.

[0043] Depend on figure 1 From the middle TLC plate, it can be seen that there are two forms of boron in calcium fructose borate, and the Rf values ​​are 0.17 an...

Embodiment 2

[0045] Taking the linoleic acid boronite mixture as an example, TLC analysis of boron was carried out.

[0046] A rapid detection method for detecting the form of boron in linoleic acid boronite mixture:

[0047] (1) Take a small amount of linoleic acid boronite mixture as the sample to be tested;

[0048](2) Prepare dilute sulfuric acid with a concentration of 1.8mol / L; weigh curcumin, dissolve it in distilled water, and the concentration of curcumin is 0.8mg / mL; slowly add the prepared dilute sulfuric acid into curcumin aqueous solution, Mix at a ratio of 90:1, shake well;

[0049] (3) Prepare developer (ethyl acetate: n-hexane = 1:19);

[0050] (4) Spot on the TLC plate, develop and develop color;

[0051] (5) Calculate R f value.

[0052] Depend on figure 2 It can be seen from the middle TLC plate that there are various forms of boron-containing compounds in the linoleic acid boronite mixture, R f =0.82 represents linoleic acid boronite, R f =0.15 or so represents...

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Abstract

The invention discloses a method for quick qualitative detection of an existing form of a boron element in a food. According to the method, firstly, a sample is pretreated, and solvent extraction is performed; a color developing agent is prepared, including 0.8-2.7 mol / L of dilute sulphuric acid and 0.8-1.3 mg / mL of curcumin, wherein the volume ratio of the two components is 84-97 to 1; a developing solvent is prepared; sample application, developing and color developing are performed on a TLC plate; a Rf value is calculated and is analyzed with a standard sample to determine the existing formof the boron element. The existing form of the boron element in the food can be quickly, simply and conveniently determined, and the method is a quick qualitative detection method. The method is a quick, safe and economic boron compound specifity detection method and can be used for united use with a traditional instrument analysis method, a boron-containing compound in a food is qualitatively and quantitatively detected, and production of positive results is avoided.

Description

technical field [0001] The invention belongs to the field of food quality and safety detection, in particular to the rapid qualitative detection of the form of boron element in boron-containing food. Background technique [0002] Boron is an essential element for the growth of higher plants. It participates in the synthesis and strength control of plant cell walls, the regulation of cell membrane potential and permeability, and the transformation of phenolic compounds. Therefore, plant-derived foods (such as soybeans and asparagus) contain relatively high amounts of boron. This type of boron is the endogenous boron naturally contained in related food raw materials and cannot be avoided. The specific function of boron in the human body has not been fully elucidated yet, but existing research results show that boron has a wide range of physiological activities, such as increasing the activity of nerve cholesterol lipolytic enzymes, reducing the activity of tumor cells, increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 汪东风孙逊袁永凯
Owner OCEAN UNIV OF CHINA