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Bone tissue repair ink, composition, scaffold, preparation method and kit

A technology of bone tissue and composition, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of affecting cell growth, easy loss of cell characteristic protein expression, etc.

Active Publication Date: 2021-11-05
REGENOVO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, in the application of bone tissue engineering, the common three-dimensional scaffold has the problem that the expression of some characteristic proteins of cells is easy to lose, which affects the growth of cells on the scaffold

Method used

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  • Bone tissue repair ink, composition, scaffold, preparation method and kit
  • Bone tissue repair ink, composition, scaffold, preparation method and kit
  • Bone tissue repair ink, composition, scaffold, preparation method and kit

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preparation example Construction

[0048] Some embodiments of the present invention provide a method for preparing bone tissue repair ink, the steps of which are as follows:

[0049] (1) A mixture of one or more biodegradable materials is provided.

[0050] (2) A mixture of one or more auxiliary molding materials is provided.

[0051] (3) Select a material formula 1 from one or more biodegradable materials in step (1), select a formula 2 from one or more auxiliary molding materials in step (2), and combine formulas 1 and 2 Slurry to form bone tissue repair ink.

[0052] Further, the biodegradable material in the bone tissue repair ink is hydroxyapatite or tricalcium phosphate, and the mass ratio of hydroxyapatite and tricalcium phosphate in the bone tissue repair ink is about 0:10-10:0 , such as 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0. The mass percentage of the mixture of hydroxyapatite and tricalcium phosphate in the bone tissue repairing ink is 50%-80%. In some preferred embodiments, the ...

Embodiment 1

[0132] This embodiment provides a scaffold for bone tissue repair, which is prepared according to the following steps:

[0133] (1) The mixed powder of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) with a mass ratio of 7:3 is used as a raw material, and a mixture of deionized water and glycerol (a volume ratio of 6: 4) is used as a solvent, sodium polyacrylate is used as a dispersant, and a vacuum defoaming machine is used for gradient centrifugal stirring for 1 h (the maximum speed is set at 3000 rpm) to prepare a slurry with a solid phase content of 38 vol%. Add collagen to the slurry as a bio-adhesive, the addition is 1wt% of the mixed powder, use a vacuum defoamer to mix for 2 hours, and use ultrasonic vibration (frequency 100Hz, time 30min, temperature 30°C), low temperature defoaming ( time 12h, temperature 4°C), to obtain bioactive ceramic slurry, the viscosity of the slurry is lower than 100Pa·S, and the solidification time in air is less than 1min.

[0134]...

Embodiment 2

[0137] This embodiment provides a scaffold for bone tissue repair, which is prepared according to the following steps:

[0138] (1) The mixed powder of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) with a mass ratio of 7:3 is used as a raw material, and a mixture of deionized water and glycerol (a volume ratio of 6: 4) is used as a solvent, sodium polyacrylate is used as a dispersant, and a vacuum defoaming machine is used for gradient centrifugal stirring for 1 h (the maximum speed is set at 3000 rpm) to prepare a slurry with a solid phase content of 38 vol%. Sodium alginate was added to the slurry as a bio-binder in an amount of 0.8wt% of the mixed powder, mixed for 2 hours using a vacuum defoamer, and degassed by ultrasonic vibration (frequency 100Hz, time 30min, temperature 30°C) and low temperature. Bubble (time 12h, temperature 4°C) to obtain bioactive ceramic slurry, the viscosity of the slurry is lower than 100Pa·S, and the solidification time in air is less ...

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Abstract

The invention relates to the field of bone tissue engineering, in particular to a bone tissue repair ink, a composition, a bracket, a preparation method and a kit. Using the bone tissue repair ink and the bioactive carrier that wraps the cells as raw materials, a bioprinter is used to print the bone tissue repair scaffold according to the preset three-dimensional structure. At the same time, the bone tissue repair ink and the bioactive carrier that encapsulates the cells are used as raw materials to realize the printing of living cells. The cells are evenly distributed on the scaffold model formed by the bone tissue repair ink, and are not easy to slide down to the bottom of the scaffold, which effectively solves the problem that the expression of certain characteristic proteins of cells is easily lost in the prior art. Facilitate the growth of cells on the scaffold. And it better simulates the growth environment of human bone cells, promotes cell proliferation, directional differentiation and specific protein expression, and is conducive to the stretching, migration, and establishment of cell connections in bone tissue scaffolds, and the formation of organic constructs.

Description

technical field [0001] The invention relates to the field of bone tissue engineering, in particular to a bone tissue repair ink, a composition, a bracket, a preparation method and a kit. Background technique [0002] Currently, bone defects caused by infection, tumor, and trauma are limited by the source of autologous bone and the specificity of bone structure, and it is difficult for traditional bone grafting techniques to prepare personalized scaffold materials according to the structure of the specific injury site, making bone defects It has always been a difficult problem in clinical treatment. This is because traditional methods (including solution casting / ion elution, electrostatic spindle, phase separation / lyophilization, in-situ forming, gas / hole-forming fiber weaving, fiber bonding, melt molding, and air pressure molding) pore method, etc.), lack of stable structure, poor mechanical properties, and residual harmful chemical solvents, it is difficult to realize the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/58A61L27/12A61L27/18B33Y70/10B33Y80/00
CPCA61L27/12A61L27/18A61L27/58A61L2430/02B33Y70/00B33Y80/00C08L67/04
Inventor 徐铭恩王玲
Owner REGENOVO BIOTECH