A preparation method of collagen/sodium alginate composite hydrogel for sustained release of sdf-1

Abstract

The invention discloses a preparation method of collagen / sodium alginate composite hydrogel for sustained release of SDF‑1, which comprises the following steps: (1), weighing 0.5g of collagen powder into a bottle filled with acetic acid solution, fully Dissolve, adjust the pH of the collagen solution to 7.2 to obtain collagen solution A; (2), weigh SA powder and slowly add it to double distilled water, wait for SA to completely dissolve after 24 hours, and obtain SA solution B; (3), weigh CaCO 3 Add the powder into double distilled water to get CaCO 3 Solution C; (4), weigh gluconolactone powder and add it to double-distilled water to obtain GDL solution D; (5), mix collagen solution A and SA solution B in equal volumes to obtain composite solution E; (6), mix the solution E, solution C and solution D are mixed according to the corresponding volume ratio and then added dropwise with SDF‑1 solution to obtain the product; the composite hydrogel has good cytocompatibility to bone marrow mesenchymal stem cells and can improve the learning and memory ability of TBI rats and neurological deficits.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of a collagen / sodium alginate composite hydrogel with sustained release of SDF-1. Background technique [0002] Traumatic brain injury (TBI) is a central nervous system defect disease with high morbidity and mortality, and the lack of adequate blood and nutrient delivery to the trauma site results in limited neural repair and regeneration. [0003] Stem cell transplantation can effectively treat liver, kidney, nerve and other damaging diseases, and promote the regeneration of cells in the damaged area. [0004] Since the 1980s, the continuous development and improvement of tissue engineering technology has brought hope for human beings to solve the problem of brain injury. Using tissue engineering technology, selecting degradable scaffold materials with good biocompatibility, and modifying the three-dimensional structure of the scaffold materi...

AI Technical Summary

Problems solved by technology

At present, researches on the combination of PLGA, PLA, HA / Lamini and other artificial synthetic materials with SDF-1 are mainly carried out at home and abroad. There is no research on the use of Col / SA scaffold materials to carry SDF-1 for TBI nerve repair and regeneration.

[0007] At the same time, it has been reported that the chemotactic effect of SDF-1 on stem cells has duality, the chemotactic homing effect is weakened when the concentration is too high, and the obvious chemotactic effect cannot be achieved when the concentration is too low

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