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Preparation method of high-purity actinomyces extract solution with antagonism

An antagonistic, high-purity technology, applied in the field of microorganisms, can solve the problems of the growth of heterophylla and tea trees being affected by pathogenic bacteria, and achieve the effects of facilitating production and storage, increasing yield, and increasing yield

Inactive Publication Date: 2019-03-01
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the deficiencies in the prior art, the present invention provides a method for preparing an antagonistic high-purity actinomycete extract, which solves the problem that the growth of Pseudostellaria Pseudostellaria and tea trees is affected by pathogenic bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preparing an antagonistic high-purity actinomycetes extract, comprising the following steps:

[0040] A: Soil collection: collect several soil samples from the land of heterophylla heterophylla and tea trees, then put the collected soil samples in a sterile envelope bag and place them in a ventilated place to dry for 1 day, then remove small stones and grass roots;

[0041] B: Sample treatment: Weigh 5g of air-dried soil sample with BSA-124S ​​precision electronic balance, then place the soil sample in a 10mL centrifuge tube, spray some sterile water on the surface of the soil sample to moisten the soil sample, the purpose It is to enable the soil sample to fully absorb microwaves during the processing process, then let it stand for 2 minutes, and then put the centrifuge tube into a water bath cup filled with 600mL tap water, the purpose is not to make the temperature of the soil too high and put it in the soil kills microorganisms;

[0042] C: Microwave t...

Embodiment 2

[0051] A method for preparing an antagonistic high-purity actinomycetes extract, comprising the following steps:

[0052] A: Soil collection: Collect several soil samples from the land of heterophylla heterophylla and tea trees, then put the collected soil samples in a sterile envelope bag and place them in a ventilated place to dry for 3 days, then remove small stones and grass roots;

[0053] B: Sample treatment: Weigh 5g of air-dried soil sample with BSA-124S ​​precision electronic balance, then place the soil sample in a 10mL centrifuge tube, spray some sterile water on the surface of the soil sample to moisten the soil sample, the purpose It is to enable the soil sample to fully absorb microwaves during the process of processing, then let it stand for 4 minutes, and then put the centrifuge tube into a water bath cup filled with 600mL tap water, the purpose is to prevent the temperature of the soil from being too high kills microorganisms;

[0054] C: Microwave treatment:...

Embodiment 3

[0063] A method for preparing an antagonistic high-purity actinomycetes extract, comprising the following steps:

[0064] A: Soil collection: collect several soil samples from the land of heterophylla heterophylla and tea trees, then put the collected soil samples in a sterile envelope bag and place them in a ventilated place to dry for 5 days, then remove small stones and grass roots;

[0065] B: Sample treatment: Weigh 5g of air-dried soil sample with BSA-124S ​​precision electronic balance, then place the soil sample in a 10mL centrifuge tube, spray some sterile water on the surface of the soil sample to moisten the soil sample, the purpose It is to enable the soil sample to fully absorb microwaves during the processing process, then let it stand for 6 minutes, and then put the centrifuge tube into a water bath cup filled with 600mL tap water, the purpose is not to make the temperature of the soil too high and put it in the soil kills microorganisms;

[0066] C: Microwave ...

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Abstract

The invention discloses a preparation method of high-purity actinomyces extract solution with antagonism and relates to technical field of microorganisms. The preparation method includes the steps ofA, soil collection ; B, sample treatment; C, microwave treatment; D, dilution; E, separation; F, screening of antagonistic bacteria; G, secondary screening; H, amplification; I, detection and J, extraction. According to the preparation method, actinomyces are extracted from soil, so that damage to radix pseudostellariae and tea tree from pathogenic bacteria can be suppressed, problems of slow growth, small fleshy root, low output and low quality of plants infected by the pathogenic bacteria, yield of plants is increase and, income of farmers is guaranteed, the problem about growth of crops canbe solved through extraction of actinomyces, the advantages of no toxin, no residues, no environmental pollution and the like are achieved, the yield of crops is increased while soil is protected, and production and storage of biological preventive preparations are facilitated.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method of a high-purity actinomycetes extract with antagonistic effect. Background technique [0002] Tea trees are native to China. They are perennial evergreen woody plants of the genus Camellia in the family Theaceae. They like shade and humidity, and they are mostly distributed in subtropical regions. Most areas in Fujian Province have lush vegetation, high mountains and many clouds, which are conducive to the growth and development of tea trees. Therefore, the tea trees in Fujian The tea planting area is expanding continuously. For a long time, Fujian's tea production ranks first in the country. Ningde City is the largest tea-producing prefecture-level city in Fujian Province, and its planting area and production account for about 1 / 3 of the province. [0003] According to relevant reports and statistics, there are more than 100 kinds of tea tree disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01P1/00A01P3/00
CPCC12N1/20C12N1/02
Inventor 叶祖云陈美霞倪建成彭成彬王泽榕甘鹏吴箭生汤钰雯余晓玲周志铭
Owner NINGDE NORMAL UNIV