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A method for preparing 4-hydroxycinnamic acid from spirulina

A technology of hydroxycinnamic acid and spirulina, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc.

Active Publication Date: 2021-08-31
厦门集大国际学术交流有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still few reports on the extraction of 4-hydroxycinnamic acid from Spirulina in domestic and foreign literature

Method used

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  • A method for preparing 4-hydroxycinnamic acid from spirulina
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  • A method for preparing 4-hydroxycinnamic acid from spirulina

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Embodiment

[0042] The invention discloses a method for preparing 4-hydroxycinnamic acid from spirulina, which comprises the following steps:

[0043] Preparation of crude extract:

[0044] Weigh 5Kg of spirulina powder and divide it into 4 parts on average, put them in 4 3L Erlenmeyer flasks, add methanol to the 2L mark and shake well, extract for 4 hours, filter with suction, and collect the filtrate; then add methanol to the residue to 2L Carry out the second leaching for 4 hours on the scale mark, suction filtration, and collect the filtrate; in this way for 5 extractions, first filter the extract with filter paper each time, and evaporate the solvent with a rotary evaporator. Redissolve in methanol, and filter again with a 0.5-micron membrane to remove insoluble matter; combine the filtrates and concentrate to obtain 85 g of crude methanol extract.

[0045] Separation and purification of methanol crude extract extract:

[0046] Take 12.86g of the crude extract of methanol and dry-m...

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Abstract

The invention discloses a method for preparing 4-hydroxycinnamic acid from spirulina, comprising the following steps: step 1, preparation of crude methanol extract; step 2, separating component T1 from methanol crude extract, Step 3, component T1 is separated to obtain component A and component B; step 4, component A is separated and purified to obtain component A1, and component B is separated and purified to obtain component B11; step 5, component A1 is separated and purified to obtain compound X , component B11 is separated and purified to obtain compound Y; step 6, the structure identification of compound X is 4-hydroxycinnamic acid, that is, 4-hydroxycinnamic acid is obtained. In the present invention, after gradually extracting by adding the extracting liquid in spirulina, and through the chromatographic separation of the reversed-phase silica gel column and the normal phase silica gel column, 4-hydroxycinnamic acid is finally obtained, and the 4-hydroxycinnamic acid isolated from spirulina is provided. A new approach to hydroxycinnamic acids.

Description

technical field [0001] The invention relates to the technical field of 4-hydroxycinnamic acid extraction, in particular to a method for preparing 4-hydroxycinnamic acid from spirulina. Background technique [0002] 4-Hydroxycinnamic acid (Coumalic acid), the chemical name is p-hydroxycinnamic acid, also known as p-4-hydroxycinnamic acid, 4-hydroxyphenylacrylic acid, which belongs to the most important kind of hydroxycinnamic acid compounds. It is widely distributed in the stems of gramineous plants, mostly in leguminous plants, and can be extracted from plants such as Haijinsha and Hedyotis diffusa. A large number of studies have shown that 4-hydroxycinnamic acid has good biological activity, and has antioxidant, antibacterial and antiviral effects. In addition, toxicology experiments have shown that 4-hydroxycinnamic acid also has choleretic function, with a wide range of safety and mild and long-lasting effects. It is clinically suitable for gallbladder and biliary dysfun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C51/47C07C59/48
CPCC07C51/42C07C51/47C07C59/48
Inventor 李健苏文金曾荣急苏国成李桂玲王力
Owner 厦门集大国际学术交流有限公司