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Artificial culture method of chestnut mycorrhizal fungus

A technology of artificial cultivation and chestnut fungus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve problems such as low survival rate, growth retardation, and death of large trees, achieving good results and simple operation Effect

Inactive Publication Date: 2019-03-19
张坤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the conventional grafting of chestnut seedlings, due to the lack of mycorrhizal fungi that can co-exist with the root system of chestnut in the seedbed or culture medium, the conventional grafted seedlings transplanted to the field have low survival rate, slow growth and development, and after 2 to 3 years There will also be death of large trees
[0005] However, the symbiotic relationship between chestnut roots and ectomycorrhizal fungi is very complicated, and it is difficult to isolate and cultivate many mycorrhizal fungi.
There have been successful reports on the mycorrhization of chestnuts, but the inoculated bacteria are all foreign strains, and the inoculation effect is very unsatisfactory.
Although Qin Ling and others also successfully collected a variety of fungal fruiting bodies from the soil in chestnut-producing areas, they did not isolate the strains that can be successfully grafted back.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for artificially cultivating Chinese chestnut mycorrhizae, comprising the following steps:

[0031] 1) Collection of fruiting bodies of wild mycorrhizal fungi

[0032] Collect fresh, strong, and moderately mature fruiting bodies of wild mycorrhizal mycorrhizal fungi from chestnut gardens over 10 years old. After removing surface debris, soak them in a pre-prepared sterile preservative for 1 to 2 minutes, remove and drain. Put it in a sterile fresh-keeping bag, seal it, take it back within 48 hours, soak it in 75% alcohol for 5 seconds for disinfection, and then wash it twice with sterile water.

[0033] Wherein, the aseptic antistaling agent is a mixture of 0.04% ascorbic acid solution and 0.02% citric acid solution in a volume ratio of 1:1.

[0034] 2) Tissue separation

[0035] Under aseptic conditions, the cleaned and sterilized fruit body is longitudinally divided into two halves, and the intersection of the cap and the stipe is cut with a scalpel to obta...

Embodiment 2

[0071] A method for artificially cultivating Chinese chestnut mycorrhizae, comprising the following steps:

[0072] 1) Collection of fruiting bodies of wild mycorrhizal fungi

[0073] In chestnut gardens over 10 years old, collect fresh, strong, and moderately mature wild mycorrhizal fruiting bodies, remove surface debris, soak in pre-prepared sterile fresh-keeping solution for 2 minutes, remove and drain, and pack In a sterile fresh-keeping bag, seal it, take it back within 48 hours, soak it in 75% alcohol for 5 seconds for disinfection, and then wash it twice with sterile water.

[0074] The aseptic antistaling agent is a mixed solution prepared with a mass concentration of 0.02% ascorbic acid solution and a mass concentration of 0.01% citric acid solution in a volume ratio of 1:3.

[0075] 2) Tissue separation

[0076] Under aseptic conditions, the cleaned and sterilized fruit body is longitudinally divided into two halves, and the intersection of the cap and the stipe is...

Embodiment 3

[0099] A method for artificially cultivating Chinese chestnut mycorrhizae, comprising the following steps:

[0100] 1) Collection of fruiting bodies of wild mycorrhizal fungi

[0101] Collect fresh, strong, and medium-mature fruiting bodies of wild mycorrhizal fungi from chestnut gardens over 10 years old. After removing surface debris, soak them in the pre-prepared sterile fresh-keeping solution for 1 minute, remove and drain, and pack In a sterile fresh-keeping bag, seal it, take it back within 48 hours, soak it in 75% alcohol for 5 seconds for disinfection, and then wash it twice with sterile water. The aseptic antistaling agent is a mixed solution prepared with a mass concentration of 0.06% ascorbic acid solution and a mass concentration of 0.03% citric acid solution in a volume ratio of 3:2.

[0102] 2) Tissue separation

[0103] Under aseptic conditions, the cleaned and sterilized fruit body is longitudinally divided into two halves, and the intersection of the cap and...

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PUM

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Abstract

The invention discloses an artificial culture method of a chestnut mycorrhizal fungus and belongs to the field of artifice culture of mycorrhizal fungi. The artificial culture method comprises the steps of 1) vertically cutting the fruiting body of the chestnut mycorrhizal fungus into two halves, and collecting the junction of cap and stipe to obtain a context tissue block; 2) placing the contexttissue block in a slant medium, culturing at 25-28 DEG C until the context tissue block grows hypha, transferring the hypha to a tube, and culturing at 25-28 DEG C until a slant is full of hypha to obtain pure hypha; 3) inoculating the pure hypha to inoculating holes of an amplification medium, and culturing at 25-28 DEG C until a culture vessel is full of hypha to obtain the chestnut mycorrhizalfungus. The artificial culture method herein is simple to perform; the fungus cultured herein is inoculated back to chestnut seedlings; mycorrhizal infection rate is up to 50.3%, and average infectionrate is 46.5%.

Description

technical field [0001] The invention belongs to the field of artificial cultivation of mycorrhizal fungi, and in particular relates to a method for artificial cultivation of chestnut mycorrhizal fungi. Background technique [0002] Mycorrhizae are the most common symbiotic phenomenon of soil microorganisms and plant compatibility. The formation of ectomycorrhizae enables trees to rely on the help of mycorrhizal fungi to improve their ability to absorb water and nutrients in the soil. The organic phosphorus decomposing enzyme secreted by ectomycorrhizal fungi can decompose and absorb organic phosphorus in litter and soil organic matter and directly transfer it to trees for use, thereby achieving the effects of drought resistance, growth promotion and disease prevention. After the ectomycorrhizal fungus infects the root system of the host plant, on the one hand it forms Hartie's net and fungus mantle; Since most ectomycorrhizal fungi do not have strict specificity to hosts, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张坤
Owner 张坤