Composite proliferation agent capable of delaying acidification of lactobacillus plantarum fermented milk
A technology of Lactobacillus plantarum and fermented milk, which is applied in the field of food processing and fermentation, and can solve problems such as slow growth, slow growth of Lactobacillus plantarum, and decreased number of viable bacteria
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Embodiment 1
[0079] Specific steps are as follows:
[0080] (1) Take raw materials according to Table 1;
[0081] (2) According to the ratio in Table 1, first premix the sweetener, thickener, emulsifier and compound proliferator, then dissolve the obtained premix in raw milk at 40°C, slowly add food flavor, and stir 30min, after mixing evenly, feed solution A was obtained;
[0082] (3) Homogenize the material liquid A, wherein the first-stage homogenization pressure is 15MPa, the second-stage homogenization pressure is 5MPa, and the homogenization temperature is 60°C. After homogenization, sterilize at 85°C for 10 minutes, and then cool to 30°C. Feed liquid B;
[0083](4) Inoculate Lactobacillus plantarum CGMCC No.5494 in feed liquid B, inoculum size 2.5×10 7 CFU / mL, the inoculation temperature is 30°C, after stirring and mixing at 200r / min for 5min, ferment at 30°C until the terminal acidity is 60-65°T, the fermentation time is 10h, and the feed liquid C is obtained;
[0084] (5) Duri...
Embodiment 2
[0095] Specific steps are as follows:
[0096] (1) Take raw materials according to Table 4;
[0097] (2) According to the proportioning in Table 4, first premix the sweetener, thickener, emulsifier and compound proliferator, then dissolve the obtained premix in raw milk at 55°C, slowly add food flavor, Stir for 15 minutes, and mix well to obtain material liquid A;
[0098] (3) Homogenize the material liquid A, wherein the first-stage homogenization pressure is 18MPa, the second-stage homogenization pressure is 2MPa, and the homogenization temperature is 70°C. After homogenization, sterilize at 95°C for 5 minutes, and then cool to 37°C. Feed liquid B;
[0099] (4) Inoculate Lactobacillus plantarum CGMCC No.5494 in feed liquid B, inoculum size 5.0×10 7 CFU / mL, the inoculation temperature is 37°C, after stirring and mixing at 100r / min for 10min, ferment at 37°C until the terminal acidity is 60-65°T, the fermentation time is 10h, and the feed liquid C is obtained;
[0100] (5)...
Embodiment 3
[0111] Specific steps are as follows:
[0112] (1) Take raw materials according to Table 6;
[0113] (2) According to the ratio in Table 6, first premix the sweetener, thickener, emulsifier and compound proliferator, then dissolve the obtained premix in raw milk at 55°C, slowly add food flavor, Stir for 15 minutes, and mix well to obtain material liquid A;
[0114] (3) Homogenize the material liquid A, wherein the first-stage homogenization pressure is 18MPa, the second-stage homogenization pressure is 2MPa, and the homogenization temperature is 70°C. After homogenization, sterilize at 95°C for 5 minutes, and then cool to 35°C. Feed liquid B;
[0115] (4) Inoculate Lactobacillus plantarum CGMCC No.6077 in feed liquid B, inoculum size 2.5×10 7 CFU / mL, the inoculation temperature is 35°C, after stirring and mixing at 100r / min for 10min, ferment at 35°C until the terminal acidity is 60-65°T, the fermentation time is 10h, and the feed liquid C is obtained;
[0116] (5) During ...
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