A method for extracting protease with hydrolytic activity from Moringa oleifera seeds
A technology of hydrolytic activity and protease, applied in the field of natural protease extraction, can solve the problem of no protease, etc., and achieve the effect of high hydrolytic enzyme activity, good proteolytic activity and strong enzyme activity
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Embodiment 1
[0073] Germination of 100g Dried Moringa Seeds and Isolation of Specific Protease
[0074] 1) Soak dried Moringa seeds in warm water: Spray 100g of dried Moringa seeds with cold water to moisten them, and then soak them in warm water at 25°C for 24 hours. The soaking process should be covered with a filter to ensure that Moringa seeds are completely immersed in warm water;
[0075] 2) Germination of Moringa seeds: Put the soaked Moringa seeds into a seedling tray covered with 3 layers of water-moistened filter paper, with an interval of 1 cm between each two Moringa seeds, then put the seedling trays into an artificial climate box, and germinate The temperature in the box is controlled at 25°C and the humidity is controlled at 70% during the period of 1 to 3 days, and the temperature in the box is controlled at 22°C and the humidity is controlled at 60% during the period of 4 to 6 days of germination;
[0076]3) Proteomic analysis of dry Moringa seeds and germinated Moringa se...
Embodiment 2
[0089] Germination of 500g Dried Moringa Seeds and Isolation of Specific Protease
[0090] Implementation process is basically the same as embodiment 1, only the following points are different:
[0091] 1) Soak dried Moringa seeds in warm water: Spray 500g of dried Moringa seeds with cold water to moisten them, then soak them in warm water at 22°C for 24 hours;
[0092] 2) Germination of Moringa seeds: Put the soaked Moringa seeds into a seedling tray covered with 3 layers of water-wetted filter paper, with an interval of 1.2cm between each two Moringa seeds, then put the seedling trays into an artificial climate box, The temperature in the box is controlled at 28°C and the humidity is controlled at 72% during the 1-3 days of germination, and the temperature in the box is controlled at 23°C and the humidity is controlled at 63% during the 4-6 days of germination;
[0093] 3) Proteomic analysis of dry Moringa seeds and germinated Moringa seeds: Germinated Moringa seeds on the ...
Embodiment 3
[0105] Germination of 1000g Dried Moringa Seeds and Isolation of Specific Protease
[0106] Implementation process is basically the same as embodiment 1, only the following points are different:
[0107] 1) Soak dried Moringa seeds in warm water: Spray 1000g of dried Moringa seeds with cold water to moisten them, and then soak them in warm water at 20°C for 24 hours;
[0108] 2) Germination of Moringa seeds: Put the soaked Moringa seeds into a seedling tray covered with 3 layers of water-wetted filter paper, with an interval of 1.5cm between each two Moringa seeds, then put the seedling trays into an artificial climate box, The temperature in the box is controlled at 30°C and the humidity is controlled at 75% during the period of 1 to 3 days of germination, and the temperature in the box is controlled at 23°C and the humidity is controlled at 65% during the period of 4 to 6 days of germination;
[0109] 3) Proteomic analysis of dry Moringa seeds and germinated Moringa seeds: ...
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