RPRM gene and novel application of expression products thereof
A gene and tumor cell technology, applied in gene therapy, microbial measurement/test, drug combination, etc., can solve the problems of increasing the radiosensitivity of tumor cells, reduce the ability of cell clone formation, change the distribution of cell cycle, and delay growth Effect
Inactive Publication Date: 2019-03-26
SUZHOU UNIV
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However, the role of RPRM in regulating DNA damage repair pathways and increasing the radiosensitivity of tumor cells has not been reported so far.
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[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0033] (1) Cell culture and irradiation
[0034] H460 cells were purchased from the Cell Bank of the Chinese Academy of Sciences. The cells were cultured in high-glucose RPMI 1640 containing 10% fetal bovine serum (Hyclone), 1 mM sodium pyruvate (Sigma-Aldrich), 100 U / ml penicillin-streptomycin (Beiyuntian Institute of Biotechnology) and 1mM HEPES (pH7.2, Nanjing Guangxuan). Different doses of radiation were given by RS2000 X-ray machine (USA), and the dose rate was 1.16Gy / min. Samples were collected and analyzed ...
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The invention relates to a RPRM gene and a novel application of expression products thereof. The invention particularly relates to a novel function of the gene Reprimo (RPRM) and the expression products in inhibiting a DNA damage repair path and increasing radiosensitivity of tumor cells, in particular to an application thereof in sensitivity enhancing auxiliary treatment of clinical tumor radiotherapy. The sensitivity enhancing effect of the RPRM to the tumor cells lies in inhibiting core upstream protein ATM of the DAN damage repair path. By reducing the expression level of the ATM, the activating level is inhibited and expression of a series of DNA damage repair related proteins inductively activated after activation is inhibited, thereby inhibiting two primary DNA damage repair paths of nonhomologous tail connection and homologous recombination and reducing the DNA damage repair ability of cells. The DNA double strands are cracked more severely and the radiosensitivity of the cellsis higher. By up-regulating the expression level of RPRM of tumor cells of patients with tumor, a purpose of increasing sensitivity enhancing of tumor radiotherapy can be achieved. The gene is further taken as an effective auxiliary means for radiotherapy of patients with tumor.
Description
technical field [0001] The invention relates to a new application of gene Reprimo (RPRM) and its expression product. More specifically, it relates to its application in tumor radiosensitization adjuvant therapy. Background technique [0002] Reprimo (RPRM) is a tumor suppressor gene activated by p53 and then involved in the regulation of cell cycle. Its protein expression product is highly glycosylated and mainly localized in the cytoplasm. As a tumor suppressor gene, RPRM participates in the regulation of cell growth and tumor cell migration through multiple mechanisms such as triggering cell cycle arrest, inhibiting cell proliferation, and promoting cell apoptosis. It regulates the dynamic balance of cells and tissues, including proliferation, migration, and cell survival. important role in the signaling pathway. Studies have shown that RPRM can inhibit the expression of Cdc2, thereby inhibiting the nuclear translocation of the cell cycle protein CyclinB1, and triggerin...
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Login to View More IPC IPC(8): C12Q1/6886A61K48/00A61P35/00
CPCA61K48/005A61P35/00C12Q1/6886C12Q2600/106C12Q2600/158
Inventor 杨红英张雅瑞张力元
Owner SUZHOU UNIV