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InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof

A molecular marker, barley technology, applied in the field of genetics and breeding, can solve the problem of undiscovered molecular marker sequence information, etc., and achieve the effects of shortening the breeding cycle, speeding up the breeding speed and reducing the breeding cost.

Active Publication Date: 2019-03-26
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no report on molecular marker sequence information of closely linked P3G and C3G synthesis genes / genetic loci in barley

Method used

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  • InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof
  • InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof
  • InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] According to the literature (Xiao-Wei Zhang, Qian-Tao Jiang, Yu-Ming Wei, et al. Inheritance analysis and mapping of quantitative trait loci (QTL) controlling individual anthocyanin compounds in purple barley (Hordeum vulgare L.) grains. Plos one, 2017, 12:e0183704) reported the main QTL PBG.ant-2H for the synthesis of P3G and C3G in colored barley grains, based on the genome information of cultivated barley Hordeum vulgare cv.Morex and wild barley AWCS079, the bioinformatics software CLCGenomics Workbench 9.0.1 was used to analyze the PBG The sequences on both sides of .ant-2H were compared, and the fragments with a continuous insertion or deletion difference of more than 50 bp in the gene sequence were screened out, and PCR amplification primer pairs were designed at both ends of the insertion or deletion position by Primer Premier 5 software, and amplified The amplification product is controlled at about 150-500bp.

[0048] Genomic DNA was extracted from young leaves...

Embodiment 2

[0055] The application test of the molecular marker BAID2H of the present invention in the selection of barley P3G and C3G synthesis main effect QTLPBG.ant-2H

[0056] The purple barley RUSSIA68 was used as the female parent and the colorless barley Gairdner was used as the male parent to obtain hybrid F1, F1 was self-crossed to obtain F2, and 120 individuals were randomly selected from F2 to form the F2 genetic verification population.

[0057] 2.1 Extraction, qualitative and quantitative analysis of anthocyanins

[0058] The grains of the F2 verification population were collected and placed in a ventilated box at 28°C for two weeks until assayed. Based on the literature (Abdel-Aal and Hucl 2007), an improved method was used to extract the anthocyanins in the grains. The specific operation process was as follows: the grains were pulverized by a ball mill and sieved through a 0.2mm sieve. The anthocyanin extract was added to the flour at a ratio of 10:1 (V / M), mixed evenly an...

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Abstract

The invention relates to the technical field of genetic breeding, in particular to an InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof. The marker with high specificity provided by the invention can accurately track the major QTL PBG.ant-2H in a purple barley parent, rapidly screen out barley varieties or lines with the P3G and C3G synthetic major QTL, and can be applied to early generation selection of barley breeding and early-stage detection of barley plant growth, and thereby the workload of breeding selection is greatly reduced, the breeding cycle is shortened, the breeding speed is increased and the breeding cost is reduced. The detection method has the advantages of simple operation, low cost and short cycle.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to an InDel molecular marker closely linked to a major QTL for synthesis of barley P3G and C3G and an application thereof. Background technique [0002] Anthocyanins are a class of water-soluble flavonoids widely found in fruits, vegetables, and grains. Anthocyanins have the ability to scavenge free oxidative radicals, which can cause oxidative stress in cells. Therefore, anthocyanins can provide various benefits to human health, especially play an important role in anti-oxidative damage and detoxification. In addition, anthocyanins also have anti-inflammatory, anti-obesity, anti-cancer and hypoglycemic effects. These biological functions make anthocyanins have considerable potential value in the fields of food industry, nutraceuticals and functional foods. Barley is widely distributed all over the world and has good adaptability in different growing environments. Barley ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张晓伟刘春吉梅时勇肖爱平魏育明江千涛杨喜爱陈小军宋志强
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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