InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof

A molecular marker, barley technology, applied in the field of genetics and breeding, can solve the problem of undiscovered molecular marker sequence information, etc., and achieve the effects of shortening the breeding cycle, speeding up the breeding speed and reducing the breeding cost.

Active Publication Date: 2019-03-26
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no report on molecular marker sequence informatio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof
  • InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof
  • InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1

[0047] According to the literature (Xiao-Wei Zhang, Qian-Tao Jiang, Yu-Ming Wei, et al. Inheritance analysis and mapping of quantitative trait loci(QTL) controlling individual anthocyanin compounds in purple barley(Hordeum vulgare L.) grains.Plos one, 2017, 12:e0183704) reported the color barley grain P3G and C3G synthesis main effect QTL PBG.ant-2H, based on the genome information of cultivated barley Hordeum vulgare cv.Morex and wild barley AWCS079, using the bioinformatics software CLCGenomics Workbench 9.0.1 to PBG The sequences on both sides of .ant-2H are compared, and the fragments with a continuous insertion or deletion difference of more than 50bp in the gene sequence between the two are screened, and PCR amplification primer pairs are designed at both ends of the insertion or deletion position through Primer Premier 5 software. The increase product is controlled at about 150-500bp.

[0048] The genomic DNA of young leaves of purple parent RUSSIA68 and ...

Example Embodiment

[0054] Example 2

[0055] Application test of molecular marker BAID2H of the present invention in selecting barley P3G and C3G to synthesize main effect QTLPBG.ant-2H

[0056] The purple barley RUSSIA68 was used as the female parent, and the colorless barley Gairdner was crossed as the male parent to obtain hybrid F1. F1 individual plants were selfed to obtain F2, and 120 individual plants were randomly selected from F2 to form the F2 genetic verification population.

[0057] 2.1 Extraction, qualitative and quantitative analysis of anthocyanins

[0058] The grains of the F2 verification population were collected and placed in a ventilated box at 28°C for two weeks until the measurement. Based on literature (Abdel-Aal and Hucl 2007), an improved method is used to extract anthocyanins from grains. The specific operation process is as follows: the grains are crushed by a ball mill, and the grains are sieved with a 0.2mm screen. Add the anthocyanin extract to the flour at a ratio of 10:1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of genetic breeding, in particular to an InDel molecular marker closely linked with barley P3G and C3G synthetic major QTL (quantitative trait loci), and application thereof. The marker with high specificity provided by the invention can accurately track the major QTL PBG.ant-2H in a purple barley parent, rapidly screen out barley varieties or lines with the P3G and C3G synthetic major QTL, and can be applied to early generation selection of barley breeding and early-stage detection of barley plant growth, and thereby the workload of breeding selection is greatly reduced, the breeding cycle is shortened, the breeding speed is increased and the breeding cost is reduced. The detection method has the advantages of simple operation, low cost and short cycle.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to an InDel molecular marker closely linked to a major QTL for synthesis of barley P3G and C3G and an application thereof. Background technique [0002] Anthocyanins are a class of water-soluble flavonoids widely found in fruits, vegetables, and grains. Anthocyanins have the ability to scavenge free oxidative radicals, which can cause oxidative stress in cells. Therefore, anthocyanins can provide various benefits to human health, especially play an important role in anti-oxidative damage and detoxification. In addition, anthocyanins also have anti-inflammatory, anti-obesity, anti-cancer and hypoglycemic effects. These biological functions make anthocyanins have considerable potential value in the fields of food industry, nutraceuticals and functional foods. Barley is widely distributed all over the world and has good adaptability in different growing environments. Barley ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张晓伟刘春吉梅时勇肖爱平魏育明江千涛杨喜爱陈小军宋志强
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap