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Detection method for predicting H7N9 susceptible populations based on HLA gene polymorphism sites and application of detection method

A technology of gene polymorphism and detection method, which is applied in the detection field of predicting H7N9 susceptible population based on HLA gene polymorphism sites, to achieve the effect of improving effectiveness and enhancing reliability

Active Publication Date: 2019-04-02
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION +2
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Problems solved by technology

[0008] HLA-A*02:06 and HLA-DRB1*12:02 are two polymorphic sites of HLA. At present, no research has shown that HLA-A*02:06 and HLA-DRB1*12:02 are related to A (H 7N 9) virus susceptibility-related

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  • Detection method for predicting H7N9 susceptible populations based on HLA gene polymorphism sites and application of detection method
  • Detection method for predicting H7N9 susceptible populations based on HLA gene polymorphism sites and application of detection method
  • Detection method for predicting H7N9 susceptible populations based on HLA gene polymorphism sites and application of detection method

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Embodiment Construction

[0023] The present invention will be further described by the following examples, but these examples should not be used to explain the limitation of the present invention.

[0024] 1. A (H 7N 9) patient and control specimen collection

[0025] The present invention collected 118 specimens of patients with A(H7N9): 32 specimens from Shanghai, 85 specimens from other provinces and cities; 131 healthy control specimens: 54 specimens from Shanghai, 77 specimens from other provinces and municipalities. Use a nucleic acid concentration detector and agarose gel electrophoresis to detect the DNA of Shanghai specimens, so that its concentration meets the minimum concentration requirements for further HLA capture and sequencing.

[0026] 2. Preliminary screening of HLA candidate loci

[0027] The first choice is to use 32 A(H7N9) patients and 54 healthy control samples from Shanghai as cohort 1, and 85 A(H7N9) patients and 77 control samples from other 6 provinces and cities as cohort ...

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Abstract

The invention discloses a detection method for predicting H7N9 susceptible populations based on HLA gene polymorphism sites. The detection method is characterized by comprising the following steps ofthe step for collecting A(H7N9) patients and healthy contrast specimens; the step of prescreening HLA candidate sites; and the step of performing verification and validation on HLA high-risk sites. The invention further discloses an application for predicting H7N9 susceptible populations based on HLA gene polymorphism sites. The application is characterized in that the polymorphism sites compriseHLA-A*02:06 and / or HLA-DRB1*12:02. A multiplex PCR target gene catching and high-flux bibasic sequencing united technique is adopted for detection and analysis on gene polymorphism of A(H7N9) avian influenza patients and healthy contrast HLA regions. Other researched large sample health contrast is used for verification, the reliability of research results of small sample group genome is improved,and the associated validity of the screened gene sites and diseases is improved.

Description

technical field [0001] The invention relates to the application field of biological analysis, in particular to a detection method for predicting H7N9 susceptible population based on HLA gene polymorphism sites and its application. Background technique [0002] Highly pathogenic avian influenza H7N9, hereinafter referred to as A(H7N9), has brought great challenges to disease prevention and control. In February 2013, a human-infecting A(H7N9) avian influenza virus was first discovered and reported in Shanghai. By August 2016, 807 patients had been diagnosed in more than three years, with a case fatality rate of 40.0%. The epidemic had three epidemic peaks. [0003] The continuous emergence of cases, high fatality rate and continuous expansion of geographical areas have brought great challenges to disease prevention and caused psychological panic to the public. Studies on the virus have found that the virus has both avian α-2,3 and human α-2,6 sialic acid receptors, and the h...

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6881C12N15/11
CPCC12Q1/6881C12Q1/6883C12Q2600/156
Inventor 张曦秦胜营舒跃龙赵雪贺林袁政安李崇山潘浩滕峥吴立梦
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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