A kind of complex microbial bacterial agent developed by heavy oil and its preparation method and application
A compound microbial agent and heavy oil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of single strains that are not resistant to environmental impact, complex preparation, etc., and achieve enhanced oil recovery , the preparation method is simple, the effect of low cost
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Embodiment 1
[0028] A kind of preparation method of the complex microbial bacterial agent of heavy oil development, comprises the following steps:
[0029] (1) Prepare liquid culture medium, wherein in this culture medium, be made by the raw material of following percentage by weight: 0.5% corn syrup, 0.5% vitamin, 0.1% KH 2 PO 4 , 0.01% NaNO 3 , 0.1% yeast powder, 0.01% benzene, 0.1% xanthan gum, and the balance is water; the pH value of the medium is adjusted to 7 and sterilized; wherein benzene can be used as both a carbon source and a solubilizer; Wherein the vitamin can be one of vitamin A, vitamin B, vitamin C, vitamin D, and vitamin E;
[0030] (2) Carry out inoculation and culture according to the ratio of the inoculation amount of Bacillus pallidum, Pantoea, and thermoanaerobic bacteria being respectively 1% of the weight fraction of the liquid culture medium, the culture time is 36h, and the culture temperature is 30°C;
[0031] (3) Mix the cultivated bacterial strains accordi...
Embodiment 2
[0033] A kind of preparation method of the complex microbial bacterial agent of heavy oil development, comprises the following steps:
[0034] (1) Prepare liquid culture medium, wherein in this culture medium, be made by the raw material of following percentage by weight: 1.5% corn syrup, 1.5% vitamin, 0.2% KH 2 PO 4 , 0.05% NaNO 3 , 0.15% yeast powder, 0.05% benzene, 0.15% xanthan gum, and the balance is water; the pH value of the medium is adjusted to 7.5 and sterilized; wherein benzene can be used as a carbon source or as a solubilizer;
[0035] (2) Carry out inoculation and culture according to the ratio of 2% of the weight fraction of the liquid culture medium according to the inoculation amount of Bacillus pallidum, Pantoea, and thermoanaerobic bacteria respectively, the culture time is 45h, and the culture temperature is 35°C;
[0036] (3) Mix the cultured bacterial strains according to 2:1:1 to obtain a composite microbial bacterial agent.
Embodiment 3
[0038] A kind of preparation method of the complex microbial bacterial agent of heavy oil development, comprises the following steps:
[0039] (1) Prepare liquid culture medium, wherein in this culture medium, be made by the raw material of following percentage by weight: 2.5% corn syrup, 2.5% vitamin, 0.5% KH 2 PO 4 , 0.1% NaNO 3 , 0.2% yeast powder, 0.1% benzene, 0.2% xanthan gum, and the balance is water; the pH value of the medium is adjusted to 8 and sterilized; wherein benzene can be used as a carbon source or as a solubilizer;
[0040] (2) Carry out inoculation and culture according to the ratio of 5% of the weight fraction of the liquid culture medium according to the inoculum amount of Bacillus pallidum, Pantoea, and thermoanaerobic bacteria respectively, the culture time is 50h, and the culture temperature is 40°C;
[0041] (3) Mix the cultured bacterial strains according to 1:1:3 to obtain a composite microbial bacterial agent.
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