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Artificial intelligence male sterility line of plant and application

A male sterile line, artificial intelligence technology, applied in applications, plant products, plant genetic improvement and other directions, can solve problems such as sterility and fertility, and achieve the effects of reducing production costs, system stability and reliability, and improving production efficiency.

Inactive Publication Date: 2019-04-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned photothermosensitive male sterile lines are greatly affected by light, temperature and other environments. Under the conditions of sudden changes and extreme weather, unexpected fertility or sterility are prone to occur, which leads to the infertility in the process of its own reproduction and seed production. There is a very high risk in

Method used

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  • Artificial intelligence male sterility line of plant and application
  • Artificial intelligence male sterility line of plant and application
  • Artificial intelligence male sterility line of plant and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the construction of carrier

[0024] 1. Construction of herbicide tolerance expression cassette

[0025]In order to construct the herbicide-tolerant expression cassette, the herbicide-resistant gene and the corresponding terminator sequence were artificially synthesized: the glyphosate-resistant gene G10evo and its terminator sequence are shown in SEQ ID NO.7; the glyphosate-resistant gene CP4 and its The terminator sequence is shown in SEQ ID NO.8; the nicosulfuron-resistant gene CYP81A9 and its terminator sequence are shown in SEQ ID NO.11; the rimsulfuron-resistant gene 3-X and its terminator sequence are shown in SEQ ID Shown in NO.12. BamHI and KpnI restriction sites were set at the 5' end and 3' of the above DNA fragments respectively.

[0026] At the same time, an operator sequence specifically binding to Tet repressor protein or Lac repressor protein was artificially synthesized: the operator sequence specifically binding to Tet repressor protein...

Embodiment 2

[0036] Embodiment 2, corn transformation

[0037] Maize transformation methods have been relatively mature, for example, Frame et al. described the method of using Agrobacterium to transform maize (Frame et al., (2002) Plant Physiol, 129:13-22). Get the Agrobacterium constructed in Example 1 (that is, the Agrobacterium containing the T-DNA vector) to scratch the plate, pick a single colony for inoculation, and prepare the Agrobacterium for transformation. Take Hi-II corn ears 8-10 days after pollination. All immature embryos (1.0-1.5 mm in size) were collected. The Agrobacterium containing the T-DNA vector was co-cultured with immature embryos for 2-3 days (22°C). Transfer the immature embryos to the callus induction medium (the medium contains 200mg / L of Timentin for killing Agrobacterium, referring to (Frame et al., (2002) Plant Physiol, 129:13-22)), Culture in dark at 28°C for 10-14 days. All calli were transferred to selection medium with a final concentration of 2 mM ...

Embodiment 3

[0039] Embodiment 3, rice transformation

[0040] The method of obtaining transgenic rice is to adopt the existing technology (Lu Xiongbin, Gong Zuxun (1998) Life Science 10: 125-131; Liu Fan et al. (2003) Molecular Plant Breeding 1: 108-115). The mature and plump seeds of "Xiushui-134" were dehulled, and callus was induced as transformation materials. Take the Agrobacterium scratch plate constructed in Example 1. Pick a single colony to inoculate and prepare Agrobacterium for transformation. The callus to be transformed is put into the Agrobacterium bacterium liquid that OD is about 0.6 (preparation of Agrobacterium bacterium liquid: Agrobacterium is inoculated to culture medium, cultivated to OD is about 0.6; Medium composition: 3g / L K 2 HPO 4 , 1g / LNaH 2 PO 4 , 1g / LNH 4 Cl, 0.3g / L MgSO 4 ·7H 2 O, 0.15g / L KCl, 0.01g / L CaCl 2 , 0.0025g / L FeSO 4 ·7H 2 0, 5g / L sucrose, 20mg / L acetosyringone, the solvent is water, pH=5.8), allow Agrobacterium to be bound to the callus...

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Abstract

The invention discloses an artificial intelligence male sterility line of a plant. A DNA fragment which comprises a protein expression frame endowing tolerance to a plant herbicide and can be combinedwith an operon to repress protein expression is imported into the artificial intelligence male sterility line of the plant. The artificial intelligence male sterility line of the plant is not limitedby photo and temperature and the system is more stable and reliable. By importing intelligence male sterility line characteristics through a biotechnology, the male sterility line is not limited by variety, and various plant resources can be fully utilized. The artificial intelligence male sterility line can show characteristics of male fertility and male sterility by spraying or not spraying some special herbicide to achieve dual purposes for two-line method seed preparation of the plant. The artificial intelligence male sterility line can be operated mechanically, so that the production cost is lowered and the production efficiency is improved.

Description

(1) Technical field [0001] The invention relates to a plant male sterile technology, in particular to the acquisition and application of an artificial intelligence male sterile line. (2) Background technology [0002] Whether in prokaryotes or eukaryotes, protein-DNA interaction is a classic way of gene expression regulation. There is a very efficient gene expression regulation mechanism in prokaryotes. When a repressor protein binds to the corresponding operator DNA, it prevents the binding of RNA polymerase, thereby inhibiting gene transcription (ReznikoffWS and JNAbelson.1980). The system in which the above-mentioned repressor protein and operator interact to regulate gene expression is the repressor protein-operator system. Currently, the repressor-operator system is widely used in prokaryotic or eukaryotic organisms to regulate gene expression. However, it has not been reported or applied for the creation of plant artificial intelligence male sterility technology. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/46A01H6/20A01H1/02
CPCA01H1/02C12N15/8274C12N15/8275C12N15/8289
Inventor 于小星张先文王东芳林朝阳沈志成
Owner ZHEJIANG UNIV
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