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A kind of Alzheimer's disease detection reagent and its application in Alzheimer's disease detection

A technology for Alzheimer's disease and detection reagents, which is applied in the field of biomedicine to achieve the effect of high repeatability and good stability

Active Publication Date: 2022-02-22
JIANGSU XIANSIDA BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of cerebrospinal fluid is quite invasive. Currently, there is no reagent on the market that can assist in the diagnosis of Alzheimer's disease by passing a blood test.

Method used

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  • A kind of Alzheimer's disease detection reagent and its application in Alzheimer's disease detection
  • A kind of Alzheimer's disease detection reagent and its application in Alzheimer's disease detection

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Experimental program
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Effect test

Embodiment 1

[0039] (1) Experimental equipment:

[0040] ABI3500dx analyzer (Applied Biosystems), PCR, centrifuge.

[0041] (2) Reagent preparation:

[0042] AD1: Mix A and B 1:1 by volume.

[0043] A: SDS (ammonium dodecyl sulfate) was dissolved in 10mM NH4HCO3, and prepared as a solution containing 5% SDS and pH 8.3

[0044] B: Mix 2.2U / µL PNGaseF and 3.33% NP-40 at a volume ratio of 1:20

[0045] AD2: Mix the same volume of 20mM APTS and 1M NaCNBH3.

[0046] AD3: 100mM NH4AC, sialidase (neuraminidase, 2mU / µL) and hydrogen peroxide according to volume 5:1:14

[0047] mix.

[0048] AD4: buffer.

[0049] (3) N-oligosaccharide chain map detection

[0050] a. Preparation of oligosaccharide chains: Take 2 µL of serum inactivated at 95°C, add 4 µL of AD1, and react at 37°C

[0051] Dry after 3h.

[0052] b. Labeling of oligosaccharide chains: Add 2 µL of reagent AD2 to the dried sample at 65°C

[0053]After 3 hours of fluorescent labeling, 200 µL of AD4 was added to terminate the rea...

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Abstract

The invention provides a detection reagent for Alzheimer's disease, including AD1: A and B are mixed according to volume 1:1, wherein A: 5.0% SDS, pH 8.3, B: 2.2U / µL PNGaseF and 3.33% NP‑40 is prepared by mixing 1:20 by volume; AD2: mix the same volume of 20mM APTS and 1M NaCNBH3; AD3: mix 100mM NH4AC, 2mU / µL sialidase and hydrogen peroxide by volume 5:1:14; AD4 : Buffer. The present invention also provides an application of a composition in a detection reagent for Alzheimer's disease, the composition is composed of NGA2F and NA2, and the composition can detect Alzheimer's disease through the ratio of NGA2F / NA2. The present invention adopts a detection method with high sensitivity, simple operation, only needs a small amount of sample, high repeatability, good stability and high throughput. Compared with the prior art, the accuracy reaches 87.7%, which is obviously superior to the existing detection method .

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an Alzheimer's disease detection reagent and its application in Alzheimer's disease detection. Background technique [0002] Alzheimer's disease, commonly known as senile dementia, is a degenerative change of the brain, mainly manifested as progressive memory and cognitive dysfunction. Currently recognized as the main pathological changes of Alzheimer's disease is the formation of β-amyloid plaques in the brain and neurofibrillary tangles mediated by tau protein. The incidence of Alzheimer's disease increases significantly with age. It usually occurs after the age of 65. The onset is not easy to detect, and the development is slow. The earliest stage often begins with progressive forgetfulness, which is often a precursor to Alzheimer's disease. The main manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, per...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6896G01N2800/2821
Inventor 陈翠英谈宗男朱宏章杨昕
Owner JIANGSU XIANSIDA BIOTECH CO LTD