Thermophilic microorganism strain used for in situ sludge reduction and applications thereof
A technology for thermophilic microorganisms and sludge reduction, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of high investment and operation costs, land and air pollution, secondary pollution, etc., and achieve convenient operation and operation management Convenience and low cost effect
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Embodiment 1
[0038] A thermophilic microbial bacterial agent for in-situ sludge reduction, the preparation method of the bacterial agent is as follows:
[0039] 1) Strain activation: inoculate 1-5 μL of frozen Bacillus subtilis into a test tube containing 5 ml of LB liquid medium, and incubate at 30-37°C, 150-180 rpm for 16h-24h;
[0040] 2) Preparation of liquid seeds: transfer the activated strains into the Erlenmeyer flask, transfer each test tube to an Erlenmeyer flask, fill each Erlenmeyer flask with 200ml LB liquid medium, and cultivate at 30-37°C, 150-180rpm for 16h-24h;
[0041] 3) Fermentation: Inoculate the prepared liquid seeds into the fermenter according to the inoculum amount of 10% (V / V) for expanded cultivation, the cultivation temperature is 30°C-35°C, pH: 6.8-7.5, rotating speed: 200rpm, fermenter pressure : 0.05Mpa, ventilation ratio: 1:1, fermentation period: 14-18h;
[0042]The fermentation medium used is: glucose 24g / L, peptone 2g / L, yeast powder 1g / L, ammonium nitra...
Embodiment 2
[0045] A thermophilic microbial bacterial agent for in-situ sludge reduction, the preparation method of the bacterial agent is as follows:
[0046] 1) Strain activation: inoculate 1-5 μL of frozen Bacillus subtilis into a test tube containing 5 ml of LB liquid medium, and incubate at 30-37°C, 150-180 rpm for 16h-24h;
[0047] 2) Preparation of liquid seeds: transfer the activated strains into the Erlenmeyer flask, transfer each test tube to an Erlenmeyer flask, fill each Erlenmeyer flask with 200ml LB liquid medium, and cultivate at 30-37°C, 150-180rpm for 16h-24h;
[0048] 3) Fermentation: inoculate the prepared liquid seeds into a fermenter with an inoculum amount of 5% (V / V) for expanded cultivation, culture temperature 30°C-35°C, pH: 6.5-7.5, initial speed: 180rpm, fermenter Pressure: 0.05Mpa, ventilation ratio: 1:1, fermentation cycle: 14-24h;
[0049] The fermentation medium used is: glucose 20g / L, peptone 5g / L, yeast powder 1g / L, ammonium nitrate 2g / L, sodium chloride ...
Embodiment 3
[0052] The aerobic tank of a sewage treatment system adopts intermittent aeration for sewage treatment. The main operating conditions are: pH6.5-8.5, temperature 20-35°C, dissolved oxygen 2-4mg / L, the sewage in the aerobic tank in the system The mud concentration is around 4000mg / L~7000mg / L.
[0053] First add the thermophilic microbial bacterial agent (the bacterial agent prepared in Example 1) according to the ratio of 0.5‰ of the volume of the aerobic pool, and then add the bacterial agent according to the ratio of 0.1‰ of the volume of the aerobic pool for 3 consecutive weeks. 1 time, that is, add the bacterial powder 4 times in total to form a stable flora.
[0054] The system has been running continuously for 60 days, and the sludge concentration is significantly reduced after adding the bacterial agent, the sludge degradation rate is 30% to 40%, and the SV (sludge settlement ratio) of the aerobic tank in the system is reduced from 85% to 48%. The mud showed better sett...
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