A molecular marker, identification method and application for identifying rice chalkiness-related gene ppdkb
A rice and gene technology, applied in the fields of genetic engineering and genetic breeding, can solve problems such as restricting popularization and application, and achieve the effects of low cost, improved quality and simple operation
Active Publication Date: 2022-03-18
江西省农业科学院水稻研究所
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However, in the current molecular marker-assisted breeding, there are only very few reports about the linkage markers of this gene, and there is no report involving the use of intragenic markers of this gene for molecular marker-assisted breeding, thus limiting the use of this gene in rice chalkiness traits. Popularization and Application of Molecular Marker Assisted Breeding
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[0038] The present invention provides a molecular marker for identifying the rice chalkiness-related gene PPDKB, the molecular marker is PPDKB-M8501, and the PPDKB-M8501 is a pair of primers, including a nucleotide sequence as shown in the sequence table SEQ ID NO.1 The upstream primer PPDKB-M8501-F and the downstream primer PPDKB-M8501-R shown in the sequence table SEQ ID NO.2, the sequence information is shown in Table 1.
[0039] The molecular marker is specifically obtained according to the following method:
[0040] Look up the clone sequence of PPDKB gene in different rice varieties by Gramene database, with the PPDKB gene sequence in japonica rice Nipponbare and indica rice 93-11 (respectively see the nucleoside in sequence table SEQ ID NO.3 and sequence table SEQ ID NO.4 acid sequence) alignment results (see figure 1 ) as the basis, select the highly conserved STS sequence at the third intron as the marker design region, use the online primer design software Primer1 t...
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The present invention relates to the fields of genetic engineering and genetic breeding, in particular to a molecular marker for identifying rice chalkiness-related gene PPDKB, an identification method and its application. The present invention provides a molecular marker for identifying rice chalkiness-related gene PPDKB. Marked as PPDKB-M8501, said PPDKB-M8501 is a primer pair, comprising a nucleotide sequence such as the upstream primer PPDKB-M8501-F shown in the sequence listing SEQ ID NO.1 and the downstream shown in the sequence listing SEQ ID NO.2 Primer PPDKB-M8501-R, the present invention utilizes the highly conserved STS region in the PPDKB gene to design a molecular marker-PPDKB-M8501 based on PCR technology, and establishes a PPDKB identification method based on the molecular marker. The molecular marker can be Applied in molecular marker-assisted breeding for rice chalkiness traits, not only is it not affected by environmental conditions, but also simple to operate and low in cost, and can be used for breeding practice of rice chalkiness traits in a simple, fast and high-throughput manner.
Description
technical field [0001] The invention relates to the fields of genetic engineering and genetic breeding, in particular to a molecular marker for identifying rice chalkiness-related gene PPDKB, an identification method and an application thereof. Background technique [0002] Rice is one of the most important food crops in my country, the planting area accounts for 30% of the food crop area, and rice is the staple food of more than 60% of the population in my country. With the continuous improvement of people's living standards, the quality of rice has attracted more and more attention. Rice quality includes appearance quality, processing quality, cooking and eating quality, and nutritional quality. One of the important indicators of appearance quality is chalkiness (including chalky grain rate and chalkiness degree). Chalkiness is the white opaque part of the rice endosperm. Due to the insufficient filling of the rice part, the starch body and protein body in the endosperm a...
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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈明亮肖叶青沈雨民熊焕金罗世友熊文涛
Owner 江西省农业科学院水稻研究所