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A nano-gold array sensor and its detection method and application

An array sensor and nano-gold technology, applied in the field of chemical sensors, can solve the problems of low sensitivity, poor specificity, and inability to detect multiple systems, and achieve the effect of good selectivity and high sensitivity

Active Publication Date: 2022-02-18
北京远大恒通科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of low sensitivity, poor specificity, and inability to detect multiplex systems in the detection of endogenous peptides in the prior art, thereby providing a high sensitivity, simple operation, low price, and simultaneous detection Nano-gold array sensor of multiple peptides and its detection method and application

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  • A nano-gold array sensor and its detection method and application
  • A nano-gold array sensor and its detection method and application
  • A nano-gold array sensor and its detection method and application

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preparation example Construction

[0069] The preparation method of gold nanoparticles is as follows: add 250 mL of chloroauric acid solution with a concentration of 1 mM into a three-necked flask, heat to boiling in a magnetic stirrer, and reflux for about 5 minutes. One of the stoppers was removed, 25 mL of 38.8 mM sodium citrate solution was quickly added to its solution, and the stopper was closed. At this time, the color of the solution changed rapidly, from pale yellow to colorless to black and finally to wine red. Continue heating to reflux for about 15 minutes, stop heating, and stir until the solution returns to room temperature, and then stop stirring. The condensed solution was passed through a filter membrane with a size of 0.45 μm. The concentration of the obtained nano-gold solution is 10nM, and the particle size is 13nm.

[0070] The preparation method of nucleotide-modified gold nanoparticles: take 100 μL of gold nanoparticles with a concentration of 10 nM, add 10 μL of C15 (5'-CCC CCC CCC CCC...

Embodiment 1

[0072] This embodiment provides a nano-gold array sensor, including a first sensing unit and a second sensing unit;

[0073] The first sensing unit includes: A1: nano-gold solution with a concentration of 10 nM;

[0074] B1: Arginine (Arg) solution, including a series of amino acid solutions at 10 concentration levels, the concentrations are 1 / 6 μM, 2 / 6 μM, 3 / 6 μM, 4 / 6 μM, 5 / 6 μM, 6 / 6 μM, 7 / 6 μM , 8 / 6μM, 9 / 6μM, 10 / 6μM;

[0075] The second sensing unit includes, A2: nucleotide-modified gold nanoparticles (AuNPs-DNA) solution, with a concentration of 10 nM;

[0076] B2: CrCl 3 Solutions, including a series of 10 concentration levels of CrCl 3 Solution, the concentration is 1 / 6 μM, 2 / 6 μM, 3 / 6 μM, 4 / 6 μM, 5 / 6 μM, 6 / 6 μM, 7 / 6 μM, 8 / 6 μM, 9 / 6 μM, 10 / 6 μM.

[0077] This embodiment also provides a method for detecting peptides using the above-mentioned array sensor, comprising the following steps:

[0078] Preparation of the first sensing unit and data measurement: take differen...

Embodiment 2

[0088] This embodiment provides a nano-gold array sensor, including a first sensing unit and a second sensing unit,

[0089] The first sensing unit includes: A1: nano-gold solution with a concentration of 10 nM;

[0090] B1: Cysteine ​​solution, including a series of amino acid solutions at 10 concentration levels, the concentrations are 1 / 6 μM, 2 / 6 μM, 3 / 6 μM, 4 / 6 μM, 5 / 6 μM, 6 / 6 μM, 7 / 6 μM, 8 / 6μM, 9 / 6μM, 10 / 6μM;

[0091] The second sensing unit includes, A2: nucleotide-modified gold nanoparticles (AuNPs-DNA(T15)) solution, with a concentration of 10 nM;

[0092] B2: CdCl 2 Solutions, including a series of 10 concentration levels of CdCl 2 Solution, the concentration is 1 / 6 μM, 2 / 6 μM, 3 / 6 μM, 4 / 6 μM, 5 / 6 μM, 6 / 6 μM, 7 / 6 μM, 8 / 6 μM, 9 / 6 μM, 10 / 6 μM.

[0093] This embodiment also provides a method for detecting peptides using the above-mentioned array sensor, comprising the following steps:

[0094] Preparation of the first sensing unit and data measurement: take differe...

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Abstract

The invention relates to the field of chemical sensors, in particular to a nano-gold array sensor and its preparation method and application. The sensor can be provided with a plurality of sensing units, so as to realize the detection of a one-element or multi-element peptide mixed system. Based on the cross-linking reaction mechanism, the first sensing unit is constructed through the competitive reaction between the peptide to be tested, gold nanoparticles and amino acids; based on the non-cross-linking reaction mechanism, metal ions can undergo complexation reactions with the peptide to be tested, and can also Neutralize the negative charges on the surface of gold nanoparticles to construct the second sensing unit. In the present invention, the UV-Vis spectrum collected by the above sensor is processed by the multidimensional partial least squares method to process the spectral data, and the quantitative analysis of the multi-component system is realized, which has the advantages of high accuracy, good stability and low detection limit.

Description

technical field [0001] The invention relates to the field of chemical sensors, in particular to a nano-gold array sensor and a detection method and application thereof. Background technique [0002] In recent decades, with the rapid development of medical science, the detection of biomolecules in body fluids has received extensive attention. In biology and clinical medicine, the measurement of tens to hundreds of peptides and proteins in clinical and biological samples with high sensitivity, specificity, reproducibility and reproducibility is increasingly required. Endogenous peptides play an important role in the homeostasis of the human body. Many endogenous peptides are in μmol·L -1 The level of concentration circulated in body fluids, the analysis of peptides at this concentration places high demands on the bioanalytical process. At present, the commonly used detection methods include immunological methods and colorimetric analysis methods. Chinese patent document CN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/27
CPCG01N21/274G01N21/314G01N2021/3155
Inventor 张卓勇黄丽娟
Owner 北京远大恒通科技发展有限公司