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Application of Acremonium sp. strain Y0997 in soil and water contamination processing

Acremonium acremonium, soil moisture content technology, applied in the field of soil microorganisms, can solve the problems of cell structure damage, damage structure, and substrate change

Active Publication Date: 2019-04-16
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] When DDT and heavy metal ions co-exist in the soil, they will compete with each other for the adsorption sites, which will limit the ability of organisms to treat the two pollutants compared with single pollutants; secondly, some heavy metal ions may interact with DDT and other organic pollutants. The combination of pollutants will lead to changes in the existing forms of the two, and changes in the substrates, which will eventually affect the effective treatment of bioremediation; finally, the simultaneous presence of heavy metal ions and DDT in the soil will affect the activity of microorganisms in the soil, such as destroying microbial activity. The structure of the cell membrane, resulting in the destruction of the cell structure

Method used

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  • Application of Acremonium sp. strain Y0997 in soil and water contamination processing
  • Application of Acremonium sp. strain Y0997 in soil and water contamination processing
  • Application of Acremonium sp. strain Y0997 in soil and water contamination processing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Taking the use of Acremonium acremonium strain Y0997 in the treatment of DDT pesticide residues in soil as an example, the steps of its use method are as follows:

[0085] The strain of Acremonium sp. was named Y0997, and was deposited in the China Center for Type Culture Collection of Wuhan University on March 14, 2016. The strain preservation number is CCTCCNO: M2016117, and the preservation address is Wuhan University, Wuhan, Hubei Province.

[0086] Its usage steps are as follows:

[0087] (1) Determination of physical and chemical properties of pesticide-contaminated soil

[0088] The pesticide pollution, nutritional status and water content in the soil were determined according to conventional methods.

[0089] (2) Pesticide residue soil pretreatment

[0090] The soil with DDT pollution of 5-10mg / kg should be pretreated, and large clods should be crushed to remove sundries.

[0091] (3) Adjust the content of C, N and P elements in the soil

[0092] Add urea, K...

Embodiment 2

[0113] Taking the use of Acremonium acremonium strain Y0997 in the treatment of DDT pesticide residues in soil as an example, the steps of its use method are as follows:

[0114] (1) Determination of physical and chemical properties of pesticide-contaminated soil

[0115] The method for measuring the physical and chemical properties of the pesticide-contaminated soil was the same as in Example 1.

[0116] (2) Pesticide residue soil pretreatment

[0117] The soil pretreatment steps with pesticide residues were the same as in Example 1.

[0118] (3) Adjust the content of C, N and P elements in the soil

[0119] Add urea, K2HPO4, and KH2PO4 sequentially to the pretreated pesticide residue soil, so that the mass ratio of C:N:P elements in the soil is 100:10:1, and the specific amount of urea added is 0.53mg / kg~1.06mg / kg, K2HPO4 is 0.069mg / kg~0.138mg / kg, KH2PO4 is 0.0535mg / kg~0.107mg / kg, add water to adjust the water content of the soil to 15%, mix well, and adjust the pH to 4....

Embodiment 3

[0131] Taking the application of Acremonium acremonium strain Y0997 in removing DDT pesticide residues in soil as an example, the steps of its application method are as follows:

[0132] (1) Determination of physical and chemical properties of pesticide-contaminated soil

[0133] The method for measuring the physical and chemical properties of the pesticide-contaminated soil was the same as in Example 1.

[0134] (2) Pesticide residue soil pretreatment

[0135] The soil pretreatment steps with pesticide residues were the same as in Example 1.

[0136] (3) Adjust the content of C, N and P elements in the soil

[0137] Add urea, K 2 HPO 4 、KH 2 PO 4 , so that the mass ratio of C:N:P elements in the soil is 100:10:1, and the specific addition amount of urea is 0.53mg / kg~1.06mg / kg, K 2 HPO 4 0.069mg / kg~0.138mg / kg, KH 2 PO 4 Add water at 0.0535 mg / kg to 0.107 mg / kg to adjust the water content of the soil to 30%, mix well, and adjust the pH to 4.5 to 6.5.

[0138] (4) Add...

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Abstract

The invention discloses application of an Acremonium sp. strain Y0997. The strain is preserved at China typical culture collection center in Wuhan University on 14th March, 2016, the preservation number is CCTCCNO:M2016117, the preservation address is Wuhan University, the city of Wuhan, the province of Hubei, and the strain is applied to processing DDT, DDD and DDE contaminations in soil, DDT contaminations in water, DDT and cadmium contaminations in soil, the removal rate of soil containing DDT reaches 86.89%, the degrading rate of soil containing DDD reaches 59.41%, the degrading rate of soil containing DDE reaches 40.80%, and the removal rate of sewage containing DDT is 75.80%, the removal rate for soil contaminated by cadmium ions and DDT reaches 60.18%, and the strain can be used forprocessing DDT, DDD and DDE contaminations in soil and contaminations of DDT-containing sewage.

Description

technical field [0001] The invention belongs to the technical field of soil microorganisms, and in particular relates to the application of acremonium acremonium. Background technique [0002] DDT (DDT), the chemical name of di-p-chlorophenyltrichloroethane, played an important role as an organochlorine pesticide in the first half of the 20th century in the fight against malaria, typhoid and other fly-borne diseases and in the prevention of agricultural pests and diseases. played an important role. DDT is chemically stable, especially in soil with a long half-life, and has strong hydrophobicity and persistence. Therefore, so far, DDT residues in soil are still found in many areas, which may affect human health through the biological chain. potential threat. [0003] When DDT and heavy metal ions co-exist in the soil, there will be mutual competition for the adsorption sites, resulting in the limitation of the ability of organisms to treat the two pollutants compared with s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/00C12N1/14C12R1/645
CPCB09C1/00B09C1/105B09C2101/00C12N1/14
Inventor 马小魁李亭亭郭文英佘旭梁健郑鹏周波李郁
Owner SHAANXI NORMAL UNIV