Method for harvesting oil-producing microalgae in urban sewage culture system on large scale by using magnetic particles

A magnetic particle and urban sewage technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low efficiency, high cost, slow speed, etc., and achieve improved separation efficiency, small secondary pollution, The effect of short separation time

Active Publication Date: 2019-04-16
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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Problems solved by technology

[0007] In view of the problems existing in the prior art, the present invention provides a method for large-scale harvesting of oil-producing microalgae in urban sewage culture systems using m

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  • Method for harvesting oil-producing microalgae in urban sewage culture system on large scale by using magnetic particles

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[0031] Example 1

[0032] A method for large-scale harvesting of oil-producing microalgae in urban sewage culture system using magnetic particles. The total nitrogen content is 50mg / L, the total phosphorus content is 5mg / L, and the urban sewage with a pH of 7 is filtered through a 50-mesh filter and enters the outdoors. Cultivate oil-producing microalgae in the racetrack pond. When the microalgae reaches a stable growth period and the concentration of the algae solution is 0.8g / L, the algae solution is introduced into the sub-velocity stirring tank through a water pump. Join Fe 3 O 4 The mass ratio with PAC is 1:3. The magnetic particle flocculant prepared by co-precipitation method is 0.5g / L, stirred at 800r / min for 3min, and stirred at 200r / min for 5min. After mixing, it flows through the magnetic separator continuously, and the algae is on the disk. The supernatant is harvested, the supernatant flows out, the hydraulic retention time is 3 minutes, and the microalgae harvest ra...

Example Embodiment

[0033] Example 2

[0034] A method for large-scale harvesting of oil-producing microalgae in urban sewage culture system by using magnetic particles. The total nitrogen content is 60mg / L, the total phosphorus content is 6mg / L, and the urban sewage with a pH of 7 is filtered through a 60-mesh filter and enters the outdoors. Cultivate oil-producing microalgae in the racetrack pond. When the microalgae reaches a stable growth period and the concentration of the algae solution is 1.0g / L, the algae solution is introduced into the sub-speed mixing tank through a water pump. Join Fe 3 O 4 The mass ratio to PAC is 1:2, 0.6g / L of magnetic particle flocculant prepared by co-precipitation-sol-gel method, stirring at 800r / min for 4min, stirring at 200r / min for 6min, and then continuously flowing through the magnetic separator after uniform mixing , The algae is harvested on the disk, the supernatant flows out, the hydraulic retention time is 4min, and the microalgae harvest rate can reach 99...

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Abstract

The invention relates to a method for harvesting oil-producing microalgae in an urban sewage culture system on a large scale by using magnetic particles. Microalgae harvesting is an important step inmicroalgae oil production, therefor, improving the microalgae harvesting method has great significance to reduce cost. The method comprises the following steps of: A, culturing the microalgae; B, adding and mixing magnetic seeds; C, carrying out solid-liquid separation; D: recovering the magnetic seeds. The algal bodies in the algal liquid with a certain concentration are completely converted intoalgal liquid with a high concentration or solid algal mud after the steps; and the separation efficiency can reach more than 95%. A magnetic flocculation method for harvesting the microalgae has theadvantages of high harvesting efficiency, small secondary pollution and short separation time, applying the magnetic method to the the large-scale microalgae harvesting field can greatly improve the separation efficiency of the microalgae, shorten the flocculation time and reduce the flocculation cost when the magnetic, most importantly, secondary pollution caused by chemical flocculation and other methods can be reduced, and technical support is provided for achieving industrialization of microalgae biodiesel.

Description

technical field [0001] The invention belongs to the field of microalgae harvesting, in particular to a method for large-scale harvesting of oil-producing microalgae in an urban sewage culture system by using magnetic particles. Background technique [0002] As an important link in the production of microalgae biodiesel, microalgae harvesting accounts for 20-30% of the total cost. High cost limits the development of microalgae biodiesel industry. There are three main reasons that restrict the efficient and low-cost harvesting of microalgae: first, the density of microalgae is similar to water, and its volume is small, and the diameter of microalgae is generally in the range of 2-20 μm, which makes it difficult to harvest microalgae; 2. The surface of microalgae cells is negatively charged in a wide pH range, and the cells repel each other. They are stable suspended in the liquid and difficult to aggregate; 3. The liquid containing microalgae has a large processing capacity. ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/01
CPCC12N1/12C12N1/20
Inventor 金文标韩松芳涂仁杰刘诗楠刘雨熹
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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