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Establishment method and application of spine date seed flavonoid component UPLC fingerprint spectrum

A fingerprint spectrum and a technology for establishing a method, applied in the field of traditional Chinese medicine identification, can solve the problems of easily lost peak information precision, detection signal baseline drift, there is no UPLC fingerprint research report on the flavonoids of jujube seed, etc., achieving stable specificity, The effect of baseline stabilization

Active Publication Date: 2019-04-16
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to good clinical curative effect and wide clinical application, the demand and price of jujube seed increase, resulting in counterfeit and adulterated products
[0003] It is reported in the literature that the quality control research of jujube kernels is mainly HPLC-ELSD fingerprints, and there are also some low-wavelength HPLC-UV fingerprints. Although the analysis information is comprehensive, the detection signal baseline drifts seriously, and peak information is easily lost and the precision is poor.
Relevant studies have also reported that the Chinese date seed medicinal material has a large amount of flavonoids. At present, there is no research report on the UPLC fingerprint of the flavonoid components of the Chinese date seed.

Method used

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  • Establishment method and application of spine date seed flavonoid component UPLC fingerprint spectrum
  • Establishment method and application of spine date seed flavonoid component UPLC fingerprint spectrum
  • Establishment method and application of spine date seed flavonoid component UPLC fingerprint spectrum

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: A method for establishing UPLC fingerprints of jujube seed flavonoids

[0026] 1. Instruments and reagents

[0027] Waters high-performance liquid chromatography (U.S. Waters company, PDA detector), Empower 3.0 chromatographic workstation. Waters ACQUITY UPLC TM I-Class ultra-high performance liquid chromatography; Waters ultra-high performance liquid phase time-of-flight high-resolution mass spectrometry system (Xevo G2-XS Q-TOF MS), UNIFI 1.8 workstation (Waters, Manchester, U.K.). One millionth analytical balance (ME204E, Mettler-Toledo); one millionth analytical balance (XP26, Mettler-Toledo company); CNC ultrasonic cleaner (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd.); constant temperature water bath (HWS28, Shanghai Yiheng Technology Co., Ltd.); ultrapure water system (Milli-Q Direct, Merck Co., Ltd.).

[0028] Spinosin (batch number: B221711108, content: 98%, China Institute for Food and Drug Control), magnolanine (batch number: 150813, conte...

Embodiment 2

[0075] Example 2: The application of UPLC fingerprints of flavonoid components in Zianzioren in the identification of the authenticity of Zianzaoren

[0076] Take other batches of jujube seed samples to be tested, extract the flavonoid components in the jujube seed samples to be tested, measure the UPLC fingerprint with the fingerprint construction method of the present invention, and calculate the similarity with the common pattern of the UPLC fingerprint of the flavonoid components of the jujube seed , the similarity > 0.98 is genuine.

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Abstract

The invention discloses an establishment method and application of a UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum of a spina date seed flavonoid component. According to the method, the spina date seed flavonoid component UPLC fingerprint spectrum capable of rapidly identifying the authenticity of the spina date seed is established, and when an unknown sample is analyzed, theunknown sample can be identified, classified and evaluated only by extracting the medicinal material flavonoid component, determining the UPLC fingerprint spectrum of the medicinal material flavonoidcomponent and calculating the similarity between the unknown sample and a common mode. The method is rapid, stable, high in specificity and stable in baseline, and a new scientific method is providedfor quality control of the spina date seed medicinal material.

Description

technical field [0001] The invention belongs to the field of identification of traditional Chinese medicines, and in particular relates to a method for establishing UPLC fingerprints of jujube seed flavonoids and its application. Background technique [0002] Ziziphus jujuba Mill.var.spinosa (Bunge) Hu exH.F.Chow is the dry mature seed of Ziziphus jujuba Mill.var.spinosa (Bunge) Hu exH.F.Chow, which is mainly distributed in Liaoning, Hebei, Henan, Shandong, Shanxi and other provinces in my country. Semen jujube mainly contains saponins, flavonoids, alkaloids, fatty acids, amino acids, polysaccharides and trace elements, and has various pharmacological effects such as sedation, hypnosis, blood pressure and blood fat reduction; The formula is used to treat symptoms such as insomnia, restlessness, palpitation and dreaminess, physical deficiency and hyperhidrosis, and thirsty wounds. Due to good clinical curative effect and wide clinical application, the demand and price of juj...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 魏梅雷桥仕梁慧程学仁童培珍王燕雄黎晓丽索彩仙彭劲源陈向东
Owner GUANGDONG YIFANG PHARMA