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Muscadine topical composition with low content of condensed tannin molecules

A topical composition, the technology of muscadine, applied in the fraction of muscadine pomace extract, in the field of reducing skin inflammation, able to solve the problems of skin damage, enlargement, etc.

Active Publication Date: 2022-03-25
SHAKLEE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The end result of the initial triggering event is the amplification of a large inflammatory response that, while designed to help the skin fight infection from invading bacteria, actually causes considerable damage to the skin

Method used

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  • Muscadine topical composition with low content of condensed tannin molecules
  • Muscadine topical composition with low content of condensed tannin molecules
  • Muscadine topical composition with low content of condensed tannin molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0178] Example 1. The MatTek EpiDerm model utilizes normal human epidermal keratinocytes that have been cultured to form multiple layers, which is a highly differentiated model of the human epidermis. Ultrastructural analysis revealed the presence of hyaline cutin granules, tonofilaments, desmosomes, and a multilayered cuticle containing an intercellular lamellar lipid layer arranged in a pattern characteristic of the epidermis in vivo. Markers of mature epidermis-specific differentiation such as protofilaggrin, K1 / K10 cytokeratin pair, involucrin and type I epidermal transglutaminase have been mapped in this model. MatTekEpiDerm is also mitotically and metabolically active.

[0179] The cytokines IL-1α and IL-6 are synthesized and stored in keratinocytes and have been identified as mediators of skin irritation and inflammation. The release of these cytokines can be measured directly in tissue culture media via a colorimetric-based enzyme-linked immunosorbent assay (ELISA)....

Embodiment 2

[0186] Example 2. Interleukin alpha (IL-1 alpha).

[0187] Exemplary topical compositions showing IL-1 production in epidermal keratinocytes of the MatTek EpiDerm model α The amount is reduced, as in the following Figure 13 and shown in Table 6. Keep MatTek full-thickness tissue at 37±2°C and 5±1% CO 2 Incubate for at least 1 hour. After this initial incubation, the assay medium was replaced with 0.9 mL of fresh medium (37±2°C). Three tissues were prepared for each test material.

[0188] Inflammatory reactions in tissues are initiated via UV irradiation (UVB). Deliver 300mJ / cm to tissue using UV light 2 dose of UVB radiation. Immediately after application of the inflammatory stimulus, 50 μL (or 50 mg for solid form) of the test material is applied directly onto the tissue surface.

[0189] Over-the-counter hydrocortisone cream was used as a positive control (only after UVB post-treatment). For "untreated" negative controls, the tissue was exposed to the inflammato...

Embodiment 3

[0197] Example 3. Prostaglandin E2 (PGE2).

[0198] Exemplary topical compositions show a reduction in the amount of PGE2 produced in epidermal keratinocytes of the MatTek EpiDerm model, as shown below Figure 14 and shown in Table 7.

[0199] A series of PGE2 standards ranging from 7.8 pg / mL to 1000 pg / mL were prepared. ELISA plates are prepared by removing any unwanted strips from the frame, remembering to designate two wells each for: total activity (TA) wells, non-specific binding (NSB) wells, maximal binding (MB) wells , and substrate blank wells (B0). Add 150 μL of tissue culture medium to the NSB wells, while adding 100 μL of medium to the B0 wells. Then add 100 μL of standards or samples to the corresponding wells. 50 μL of the PGE2 alkaline phosphatase conjugate was added to each well used (except TA and B0). Next, add 50 µL of PGE2 alkaline phosphatase antibody solution to each well (except TA, NSB, and SD wells). Cover the plate and incubate at 2-8°C for 18-...

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Abstract

The present invention discloses a method for decolorizing muscadine pomace grape extract by processing the clarified extract by ultrafiltration with a 500‑5000 kDa microfiltration membrane to obtain a first permeate from which flavor components have been removed . The retentate was ultrafiltered through a 25-100 kDa ultrafiltration membrane to obtain a second permeate and a second retentate. The polymeric condensed tannins are removed in the second retentate, which is a depigmented extract with increased levels of polyphenols and reduced levels of sugars and condensed tannins compared to the first retentate. The depigmented extract may be incorporated into skin care compositions which may also contain beta-glucan and grape seed extract. Skin care compositions can be applied to the skin to maintain healthy skin without discoloration. In some embodiments, the composition is used to reduce inflammation in the skin, including inflammation resulting from exposure to ultraviolet radiation. Disclosed is the inhibition of anti-inflammatory mediators in skin cells by applying to the skin a topical composition comprising depigmented muscadine (Vitis rotundifolia) pomace solvent extract, β-glucan, and grape seed extract. Methods of producing and inhibiting damage caused by ultraviolet radiation.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Application No. 62 / 364,222, filed July 19, 2016, and U.S. Provisional Application No. 62 / 505,543, filed May 12, 2017, which are incorporated by reference in their entirety This article. technical field [0003] The present disclosure relates to grape pomace extracts, such as muscadine (Vitis rotundifolia) pomace solvent extracts, grape pomace extraction processes, and obtaining grape pomace extracts, such as muscadine, suitable for use in skin care products Method for fractionation of grape pomace extract. The present disclosure also relates to topical compositions comprising depigmented muscadine pomace solvent extract, beta-glucan, and grape seed extract, which reduce inflammation when applied to the skin, and to the use of such topical compositions to reduce skin inflammation Methods. Background technique [0004] Grape extract is known to have beneficial anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K36/87A61K9/00A61Q19/00A61Q17/04
CPCA61K36/87A61K8/368A61K8/42A61K8/498A61K8/671A61K8/676A61K8/678A61K8/73A61Q17/04A61Q19/00A61K2800/522A61P17/00A61P17/16A61P17/18A61P29/00A61P39/06A61P43/00A61K9/0014
Inventor 劳勒尔·A·费舍克里斯托斯·基鲁罗恩·弗洛雷斯特奥多罗·安尼罗森吉·C·帕克
Owner SHAKLEE CORP
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