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Drug carrier and preparation method thereof

A drug and carrier technology, applied in the field of drug carrier and its preparation, to achieve the effect of wide source, good stability and low toxicity

Active Publication Date: 2021-01-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, acrylic resin nanospheres, chitin microspheres, collagen microspheres and other carriers have been developed at home and abroad, but whey protein nanomaterials have not been used as drug carriers. Therefore, new nanomaterials are prepared from whey protein Drug carrier, targeted therapy for cancer still to be developed and developed

Method used

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  • Drug carrier and preparation method thereof
  • Drug carrier and preparation method thereof
  • Drug carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] The characterization of different nanocarrier particles of embodiment 1

[0102] 1. Experimental method

[0103] Test Drugs and Reagents

[0104] Ultrapure water, α-lactalbumin (α-lactalbumin, sigma), proteolytic enzyme, tris(Tris, sigma), HCL, anhydrous CaCl 2 , 3% uranyl acetate dye, absolute ethanol, DMSO, fluorescent dye fat-soluble Cy5, doxorubicin hydrochloride (DOX`HCl), 25% glutaraldehyde aqueous solution.

[0105] laboratory apparatus:

[0106]Beaker, 1cm small magnet, ear washing ball, constant temperature magnetic heating stirrer, 0.22μm polyethersulfone syringe filter, 1mL sterile syringe, 220kv biological transmission electron microscope (TEM), Malvern laser particle size analyzer, high-precision analytical balance , pH meter, high-speed refrigerated centrifuge, vacuum freeze dryer, ultrapure water meter, cell disruptor, multi-functional microplate reader, vertical vortexer, drying oven, pointed tweezers, 300 mesh copper mesh, ice maker, Vortex instrume...

Embodiment 2

[0142] Example 2 Investigation of Different Nanoparticles at the Cell Level

[0143] 1. Experimental method

[0144] Experimental drugs and reagents:

[0145] Ultrapure water, α-lactalbumin (α-lactalbumin, sigma), proteolytic enzyme, tris (Tris, sigma), HCl, DOX (doxorubicin hydrochloride), agarose, sucrose, anhydrous CaCl 2 , 25% glutaraldehyde, RPMI 1640 medium, RPMI DMEM medium, fat-soluble Cy5, 4% paraformaldehyde, fetal bovine serum, penicillin-streptomycin, AlexaFluor488-Phalloidin, Hochest33342, OCT (polyethylene glycol and poly water-soluble mixture of vinyl alcohol), TritonX-100, BSA, CCK-8, TUNEL kit.

[0146] laboratory apparatus:

[0147] Beaker, blade, magnet, constant temperature magnetic heating stirrer, 0.22μm polyethersulfone syringe filter, high-precision analytical balance, pH meter, high-speed refrigerated centrifuge, vacuum freeze dryer, ultrapure water instrument, cell disruptor, Drying box, pointed tweezers, ice maker, vortex instrument, laser confoc...

Embodiment 3

[0208] Example 3 Investigation of Different Nanoparticles at the Animal Level

[0209] 1. Experimental method

[0210] Experimental materials and medicines:

[0211] Ultrapure water, α-lactalbumin (α-lactalbumin, sigma), proteolytic enzyme, tris (Tris, sigma), HCl, DOX (doxorubicin hydrochloride), sucrose, anhydrous CaCl 2 , 25% glutaraldehyde, RPMI 1640 medium, RPMI DMEM medium, fat-soluble DIR, fat-soluble Cy5, 4% paraformaldehyde, fetal bovine serum, penicillin-streptomycin, OCT (polyethylene glycol and polyvinyl alcohol water-soluble mixture), BALB / C mice, Kunming mice (KM mice).

[0212] laboratory apparatus:

[0213] Beaker, blade, magnet, constant temperature magnetic heating stirrer, 0.22μm polyethersulfone syringe filter, high-precision analytical balance, pH meter, high-speed refrigerated centrifuge, vacuum freeze dryer, ultrapure water instrument, cell disruptor, Drying box, pointed tweezers, ice maker, vortex instrument, laser confocal scanning microscope, mult...

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Abstract

The invention proposes a drug carrier, the drug carrier is a spherical carrier formed by several whey protein nanotubes, the diameter of the spherical carrier is 1-3 μm, and the pore diameter of the spherical carrier is 50-100 nm.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular, the invention relates to a drug carrier and a preparation method thereof. Background technique [0002] A large number of small-molecule drugs currently used in clinical treatment have solubility problems and low bioavailability due to hydrophobicity, which greatly limits their effective and extensive clinical applications. In order to solve these problems, researchers are trying to find a perfect drug delivery strategy to improve solubility, improve drug bioavailability, reduce drug side effects, protect drug activity, prolong circulation time, etc. New drug delivery systems are effective means to solve such problems, such as transdermal drug delivery systems (Transdermal Drug Delivery Systems, TDDS), bioadhesion drug delivery systems (Bio-adhesion Drug Delivery System, BDDS), nano drug delivery systems, etc. (Nano Drug Delivery System, NDDS), biological chip (Biologica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K9/51A61K47/18A61K31/704A61P35/00
CPCA61K9/06A61K9/5123A61K9/5169A61K31/704
Inventor 李媛鲍威尔王泓月
Owner CHINA AGRI UNIV