Device and method for enriching and culturing anaerobic ammonium oxidation bacteria at low inoculation level

Abstract

The invention discloses a device and a method for enriching and culturing anaerobic ammonium oxidation bacteria at a low inoculation level, and belongs to the technical direction of treatment of high-concentration ammonia-nitrogen wastewater in the field of biological treatment of environmental engineering wastewater. An up-flow reactor is taken as a main body, the anaerobic ammonium oxidation bacteria are enriched and cultured at the low inoculation level by means of partial nitrification coupled anaerobic ammonia oxidation, and the low inoculation level means that the sludge concentration contributed by anaerobic ammonia oxidation seed sludge in the reactor is relatively low after inoculation, namely 300-400 mg / L. The traditional method cannot effectively hold the anaerobic ammonium oxidation bacteria as the sludge flows up with nitrogen, and the quantity demand of the seed sludge is relatively high. The provided method overcomes the defects of the traditional method specifically asfollows: 1, the activity of the cultured granule sludge is high, the structure is stable, and the reactor can stably operate under a high-load condition; 2, an undercurrent at the top part flows out,so that the problem that the anaerobic ammonia oxidation granule sludge is unlikely to be held is solved effectively; 3, starting the reactor at the low inoculation level facilitates the popularization and application of a project; and 4, the method and the device are simple and feasible, and low in cost.

Description

technical field [0001] The invention relates to a device and method for enriching and cultivating anammox bacteria at a low inoculation amount, and belongs to the technical field of high-concentration ammonia nitrogen wastewater treatment in the field of environmental engineering wastewater biological treatment. Background technique [0002] With the increasing development of sewage treatment technology, new treatment processes have gradually become a research hotspot. As an ultra-efficient denitrification technology, anaerobic ammonium oxidation has attracted the attention of researchers. [0003] Anammox bacteria are a kind of highly efficient autotrophic bacteria that are ubiquitous in nature and can use nitrate nitrogen as an electron acceptor to oxidize ammonia nitrogen into nitrogen gas. The reaction equation is: [0004] NH 4 + +1.32NO 2 - +0.066HCO 3 - +0.13H + →1.02N 2 +0.26NO 3 - +0.066CH 2 o 0.5 N0.15+2.03H 2 o [0005] Due to the special metabolic ...

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Problems solved by technology

For example: SBR technology is cumbersome and cannot continuously feed water. When the sludge properties are not good, the effluent is turbid and sludge is lost; biofilm reactors can start quickly under low load conditions, but cannot withstand high loads; upflow type When the rising flow rate of the anaerobic sludge bed reactor is too high, the sludge layer is easy to collapse. When the rising flow rate is low, good hydraulic screening conditions cannot be obtained, which is not conducive to sludge growth; The mud-water separation effect is often poor, the sludge loss is serious, and it is difficult to collect the sludge after it is lost, which makes it difficult for the anammox bacteria to effectively persist in the reactor, making the start-up time of the anammox process longer; after the process is successfully started, Sludge flotation leads to serious loss of anammox bacteria; the above method

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