Preparation method for extracting and purifying mangiferin from mango leaves
A technology of mangiferin and mango leaf is applied in the field of preparation of extracting and purifying mangiferin, which can solve the problems of cumbersome process and achieve the effects of simple process, low cost and less solvent.
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[0020] The purpose of this invention is to provide a kind of preparation method of mangiferin.
[0021] The purpose of the present invention is achieved in this way, using mango leaves as raw material, through the steps of alcohol reflux extraction, concentration, cooling and filtration, to obtain mangiferin, which specifically includes the following steps:
[0022] (1) Alcohol reflux extraction: Coarsely crush mango leaves, add ethanol with a mass concentration of 50%-100% at a material-to-liquid ratio of 1:5-1:10 in kg / L, reflux extraction for 3-4 hours, filter, and remove the residue Repeat the extraction once more, filter, and combine the filtrate twice;
[0023] (2) Concentration: Concentrate under reduced pressure at 60-70°C to 1 / 5-1 / 20 volume to obtain a concentrated solution;
[0024] (3) Cooling and filtering: After the concentrated solution is cooled, a large amount of solids are precipitated, filtered, and the filter cake is washed with 50%-100% ethanol, and the ma...
Embodiment 1
[0030] (1) Alcohol reflux extraction: Weigh 1kg of mango leaves, coarsely crush them, add ethanol with a mass concentration of 50%-100% in kg / L with a material-to-liquid ratio of 1:5-1:10, and extract under reflux for 3-4 hours. Filtration, the slag was extracted again, filtered, and the second filtrate was combined;
[0031] (2) Concentration: Concentrate under reduced pressure at 60-70°C to 1 / 5-1 / 20 volume to obtain a concentrated solution;
[0032] (3) Cooling and filtering: After the concentrated solution is cooled, a large amount of solids are precipitated, filtered, and the filter cake is washed with 50%-100% ethanol, and the mangiferin is obtained after draining.
[0033] Table 1
[0034]
Embodiment 2
[0036] The prepared mangiferin was subjected to NMR, and the data are as follows: 1 H-NMR (400 MHz, Pyridine-d 5 ) δ: 14.57 (1H,s, 1-OH), 8.07 (2H, br s, 6,7-OH), 7.14 (1H, s, H-8), 6.61(1H, s, H-5), 6.37(1H, s, H-4), 5.82 (1H, d, J = 9.6Hz, H-1″); 13 C-NMR (100 MHz, Pyridine-d 5 ): 180.1 (C-9), 165.1 (C-3), 163.0 (C-1), 157.4 (C-4a), 155.8 (C-6), 151.9 (C-10a), 145.3 (C-7) , 112.0 (C-8a), 109.1(C-8), 108.6 (C-2), 103.2 (C-5), 102.6(C-9a), 94.1(C-4), 82.7 (C-5′) , 80.5 (C-3′), 75.3 (C-1′), 72.6 (C-4′), 71.8 (C-2′), 62.7 (C-6′). The above spectral data are consistent with the literature [1] The reports were basically consistent, so the compound was determined to be mangiferin.
[0037] 1. Fang Qianbo, Qin Kunming, Pan Yang, et al. Study on the chemical constituents of Baihe Zhimu Decoction[J]. Chinese Herbal Medicine, 2010, 41(4): 517-520
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