Breeding method and application of high-yield bacteriocin lactic acid bacteria
A technology of lactic acid bacteria and bacteriocin, applied in biochemical equipment and methods, treatment of microorganisms with electricity/wave energy, treatment of enzymes with electricity/wave energy, etc. Narrow spectrum, low bacteriocin production and other problems, to achieve the effect of increasing practicability and economy, enhancing acid-base stability, and increasing metabolites
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Embodiment 1
[0033] The breeding method of a strain of bacteriocin-producing lactic acid bacteria is to carry out mutagenesis treatment on lactic acid bacteria, and then screen and measure antibacterial substances to finally obtain bacteriocin-producing lactic acid bacteria; the above-mentioned mutagenesis treatment adopts the method of ultraviolet-microwave compound mutagenesis ; The above-mentioned strain culture solution for ultraviolet mutagenesis contains sodium nitrophenolate and diethylene glycol. The lactic acid bacteria obtained by this method can enter the logarithmic growth phase in advance, the metabolites increase, and are resistant to low temperature and high temperature environments. Molds have a certain inhibitory effect, and can be degraded by proteases in animals, energy saving, environmental protection, and low cost, and are suitable for large-scale food, medicine, feed and other fresh-keeping and anti-corrosion and biological control fields.
[0034] The conditions of u...
Embodiment 2
[0046] The breeding method of a high-yield bacteriocin lactic acid bacteria, its specific steps are as follows:
[0047] 1) Isolate the original strain for mutagenesis from sauerkraut, culture it to the middle and late logarithmic growth period, and then place it in the MRS liquid medium containing sodium nitrophenolate and diethylene glycol. The contents were 0.012g / L and 0.006g / L respectively, and then cultured and prepared to make the concentration of viable bacteria 10 8 The cfu / mL starting strain suspension was placed under a UV lamp with a power of 30W and irradiated for 100s to complete the UV mutagenesis;
[0048] 2) Take the strain after ultraviolet mutagenesis and insert it into MRS liquid medium with 5% inoculation amount, cultivate it at 35°C until the middle and late logarithmic growth period, then centrifuge to take the supernatant, and use the Oxford cup method to carry out the antibacterial test , the diameter of the inhibition zone is selected larger than the...
Embodiment 3
[0055] A breeding method for high-yielding bacteriocin lactic acid bacteria, wherein the microwave mutagenesis steps are as follows: place the screened strain in MRS liquid medium containing 0.05mM tetramethylguanidine and 0.02mM hippuric acid, then cultivate and prepare live bacteria Concentration is 10 8 The cfu / mL starting strain suspension was irradiated in a microwave with a power of 700W for 4min, and it was taken out every 8s and placed in an ice bath for 4s until the end of the irradiation; the strain after microwave mutagenesis was inoculated with 5% Inject a large amount of MRS liquid medium into the MRS liquid medium, cultivate it to the middle and late logarithmic growth period at 35°C, centrifuge to take the supernatant, and use the Oxford cup method to carry out the antibacterial test, and select the strain whose inhibition zone diameter is larger than the starting strain, that is, Mutagenesis completed strain; the tetramethylguanidine and hippuric acid added to ...
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