Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of anti-emulsification preparation method of plant source copper chlorophyllin sodium salt

A copper chlorophyllin sodium salt and anti-emulsification technology, which is applied in the field of anti-emulsification preparation of plant-source copper chlorophyllin sodium salt, can solve the problems of not being able to effectively reduce the content of ginkgolic acid, affecting product preparation and purification, and not being able to effectively reduce ginkgolic acid. Excellent chroma and transparency, realize comprehensive utilization and reduce content

Active Publication Date: 2020-04-14
SPEC CHEM IND INC
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of copper chlorophyllin sodium salt by using ginkgo leaves not only needs to control the content of free copper, but the ginkgolic acid in the ginkgo leaf extract is the main harmful impurity in the product, which is not easy to be separated, which greatly affects the preparation and purification of the product
[0004] Chinese patent CN 108451988A discloses a method for preparing sodium copper chlorophyllin by enzymatic hydrolysis of ginkgo leaves. This method requires the use of a large amount of protease and cellulase, and cannot effectively reduce the content of ginkgolic acid in the product, resulting in too many impurities in the product , cloudy when dissolved in water
And this method can not effectively reduce the ginkgolic acid in the system (20-50ppm)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Preparation of Ginkgo Leaf Extract

[0030] Take 100 grams of Ginkgo biloba leaves and grind them, and use 90% ethanol to reflux for 3 hours for the first extraction, and the temperature of the water bath is 90 ° C, and filter through gauze; Filter and set aside. Concentrate under reduced pressure to an extract, add water to dissolve, and centrifuge to obtain a black sticky substance.

[0031] 2) Petroleum ether phase extraction for impurity removal

[0032] Add 100ml of 95% ethanol and 400ml of petroleum ether to the aforementioned extract, stir for 0.5-1.5 hours, filter under reduced pressure, transfer to a separatory funnel, let stand, and a clear boundary layer can be seen, discard the lower alcohol phase. Then add 70% ethanol to stir and wash with alcohol for 30 minutes, let stand to separate layers, and discard the lower alcohol phase. Alcohol washing was carried out twice.

[0033] Then add 62% ethanol and 0.15% (m / V) sodium hydroxide to the petroleum e...

Embodiment 2

[0046] 1) Preparation of Ginkgo Leaf Extract

[0047] Take 100 grams of Ginkgo biloba leaves and grind them, and use 90% ethanol to reflux for 3 hours for the first extraction, and the temperature of the water bath is 90 ° C, and filter through gauze; Filter and set aside. Concentrate under reduced pressure to an extract, add water to dissolve, and centrifuge to obtain a black sticky substance.

[0048] 2) Petroleum ether phase extraction for impurity removal

[0049] Add 100ml of 95% ethanol and 400ml of petroleum ether to the aforementioned extract, stir for 0.5-1.5 hours, filter under reduced pressure, transfer to a separatory funnel, let stand, and a clear boundary layer can be seen, discard the lower alcohol phase. Then add 60% ethanol to stir and wash with alcohol for 30 minutes, let stand to separate layers, and discard the lower alcohol phase. Alcohol washing was carried out twice.

[0050] Then add 60% ethanol and 0.10% (m / V) sodium hydroxide to the petroleum e...

Embodiment 3

[0063] 1) Preparation of Ginkgo Leaf Extract

[0064] Take 100 grams of Ginkgo biloba leaves and grind them, and use 90% ethanol to reflux for 3 hours for the first extraction, and the temperature of the water bath is 90 ° C, and filter through gauze; Filter and set aside. Concentrate under reduced pressure to an extract, add water to dissolve, and centrifuge to obtain a black sticky substance.

[0065] 2) Petroleum ether phase extraction for impurity removal

[0066] Add 100ml of 95% ethanol and 300ml of petroleum ether to the aforementioned extract, stir for 0.5-1.5 hours, filter under reduced pressure, transfer to a separatory funnel, let stand, and a clear boundary layer can be seen, discard the lower alcohol phase. Then add 95% ethanol, stir and wash with alcohol for 30 minutes, let stand to separate layers, and discard the lower alcohol phase. Alcohol washing was carried out twice.

[0067] 3) saponification

[0068] Add a 95% alcohol solution with a sodium hyd...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
absorbanceaaaaaaaaaa
absorbanceaaaaaaaaaa
Login to View More

Abstract

The invention discloses an anti-emulsification preparation method of a plant source chlorophyllin copper complex sodium salt. According to the method provided by the invention, ginkgo biloba leaves are used as a raw material, alcohol extraction is performed to obtain black extract, forward and reverse extraction are carried out by utilizing different concentrations of an ethanol solvent and a petroleum ether solvent, so that a phenomenon of emulsification layering is reduced, the content of ginkgolic acid in the obtained chlorophyllin copper complex sodium salt is less than 1 ppm, and the E405nm absorbance is >= 600.

Description

technical field [0001] The invention relates to the fields of plant extraction and chemistry, in particular to an anti-emulsification preparation method of plant source copper chlorophyllin sodium salt. Background technique [0002] Sodium copper chlorophyllin is a pigment product from natural sources, including animal sources (such as silkworm excrement) and plant sources (such as leaves of alfalfa, bamboo, ginkgo, etc.), and chlorophyll is converted into chlorophyll through saponification during production , then acidify copper to obtain chlorophyll cupric acid, and neutralize chlorophyll cupric acid with sodium hydroxide to obtain water-soluble sodium chlorophyll cuprate. GB 26406-2011 discloses the use of organic solvents to extract silkworm excrement, saponification and copper substituted sodium copper chlorophyllin as a food additive standard, requiring strict control of free copper content during production, and absorbance E405nm ≥ 568. With the gradual improvement o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/22C09B61/00C09B67/54
Inventor 童莉查建生程若兰李新岗
Owner SPEC CHEM IND INC