Method for efficiently synthesizing resveratrol and coproducing luteolin by bioconversion of peanut waste with composite microbial agent and application
A compound bacterial agent and biotransformation technology, which is applied in the field of agricultural application microorganisms, can solve the problems that there are no patents for the production methods of resveratrol and luteolin, and achieve the effect of efficient utilization
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Embodiment 1
[0037] A method for biotransformation of peanut waste by compound microbial agents to efficiently synthesize resveratrol and co-produce luteolin, the process is as follows figure 1 As shown, including the following steps:
[0038] (1) Expand the cultivation of Cellulomonas, Aspergillus niger and Phomasoma on the corresponding medium, the process is as follows figure 2 Shown. The specific steps are: ① Wash the slope with sterile normal saline, and shake it in a sterile Erlenmeyer flask to form a bacterial suspension; ② Inoculate the slope culture in the seed bottle, cultivate at 30℃, 200r / min for 2 to 3 days to obtain seeds Liquid; ③10% of the seed liquid is inoculated into the fermentation bottle and expanded for 3 to 4 days to obtain the fermentation liquid, and the microbial compound microbial agent is prepared according to the ratio shown in Table 2.
[0039] Table 2 Example 1 Compound Bacteria Proportion (mL)
[0040]
[0041] (2) After crushing the peanut stalks, pass through ...
Embodiment 2
[0048] A method for biotransformation of peanut waste with compound inoculants to efficiently synthesize resveratrol and co-produce luteolin, including the following steps:
[0049] (1) Select cell Vibrio, Aspergillus oryzae, and Ceratobasidiomycetes to inoculate the corresponding medium for amplifying culture to obtain the seed liquid. The process is as follows figure 2 As shown, the specific steps are the same as in Example 1. According to Table 4, mix the required microbial compound inoculants.
[0050] Table 4 Example 2 Compound Bacteria Proportion (mL)
[0051]
[0052] (2) The peanut shells after the peanut oil extraction process are harvested, washed and dried, crushed with a grinder, and passed through a 60-mesh sieve. Weigh 100g of peanut shell powder, and then add water according to the material-to-liquid ratio of 20:1 to adjust C / N The ratio is 20:1~30:1, with 0.5~1.5g / L inorganic salt for high pressure steam sterilization to prepare liquid fermentation medium.
[0053] (...
Embodiment 3
[0056] A method for bio-transformation of peanut waste by compound bacteria to synthesize resveratrol and co-produce luteolin. Peanut seeds are peeled to obtain peanut red coat, dried and crushed and sieved. The content of cellulose and lignin in peanut red coat is almost equal to Therefore, in this example, only two species of Trichoderma harzianum and Alternaria alternata were selected for liquid fermentation culture.
[0057] Specific steps are as follows:
[0058] (1) Inoculate Trichoderma harzianum and Alternaria alternata on the corresponding medium to enlarge the culture to obtain seed liquid.
[0059] (2) Weigh 10-20g of peanut red coat, add an appropriate amount of inorganic salt medium, adjust the C / N ratio to 20:1-30:1, and perform high-pressure steam sterilization to obtain a liquid fermentation medium.
[0060] (3) The Trichoderma harzianum and Alternaria alternata are configured into corresponding microbial compound agents according to 1:1, 1:2, 1:2.5, 1:3, 2:1, 2.5:1 an...
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