A compound with antibacterial activity and its preparation method and application
An antibacterial activity and compound technology, applied in the field of biological antibiotics, can solve the problems of stability limitation and no antibacterial activity of glycoside compounds, and achieve the effects of short production cycle, stable product and high content
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Embodiment 1
[0059] A preparation method of a compound with antibacterial activity, comprising the steps of:
[0060] (1) Inoculate Bacillus amyloliquefaciens into LB broth medium, culture at 37°C and 180rpm for 16 hours to obtain seed solution;
[0061] The prepared seed solution was inoculated in the fermentation medium with an inoculation amount of 5% (volume percentage), and cultivated at 30°C and 200rpm for 48 hours to obtain a fermentation solution;
[0062] The fermented liquid of Bacillus amyloliquefaciens was centrifuged, the supernatant was taken, concentrated by rotary evaporation to 10% of the original volume, and then extracted with methanol at a volume ratio of 1:1 to obtain the extract; the antibacterial activity was detected, and the results were as follows: Figure 9 shown;
[0063] (2) After the extract obtained in step (1) is dried, the active compound crude extract is obtained, and the obtained crude extract is separated by 200 mesh silica gel column chromatography, th...
experiment example
[0070] Antibacterial experiment 1
[0071] (i) Using the crude extract in Example 1, dilute it with sterile water so that the content of the crude extract is in the range of 35.85-358.5 mg / mL;
[0072] (ii) Add 100 μL of Staphylococcus aureus, Salmonella pullorum, Salmonella enteritidis, and Pasteurella multocida pre-cultured for 14-16 hours in each EP tube, centrifuge, discard the supernatant to retain the above-mentioned pathogenic bacteria, And add fresh 950μL LB medium, mix well;
[0073] (iii) Corresponding to the number, add 50 μL of the sample in (i) respectively, and set up a control group;
[0074] (iv) After the preparation is completed, put it into a shaker and cultivate it for 14-16h;
[0075] (v) After the culture is completed, use a microplate reader to detect the OD 600 ;
[0076] After testing, the glycoside compound prepared in Example 1 has a significant inhibitory effect on Staphylococcus aureus, Salmonella pullorum, Salmonella enteritidis, and Pasteurel...
Embodiment 2
[0083] A preparation method of a compound with antibacterial activity, comprising the steps of:
[0084] (1) Inoculate Bacillus amyloliquefaciens into LB broth medium, and culture at 35°C and 200rpm for 14 hours to obtain seed solution;
[0085] The prepared seed solution was inoculated in the fermentation medium with an inoculation amount of 3% (volume percentage), and cultured at 32°C and 180rpm for 52 hours to obtain a fermentation solution;
[0086] The fermented liquid of Bacillus amyloliquefaciens was centrifuged, the supernatant was taken, concentrated to 8% of the original volume by rotary evaporation, and then extracted with methanol at a volume ratio of 1:1 to obtain the extract; the antibacterial activity was detected, and the results were as follows: Figure 9 shown;
[0087] (2) After the extract obtained in step (1) is dried, the active compound crude extract is obtained, and the obtained crude extract is separated by 200-mesh silica gel column chromatography, t...
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