A kind of apple rootstock tissue culture seedling rootless rapid transplanting method
A technology for tissue culture seedlings and rootstocks, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of low transplanting survival rate and long rooting time
Active Publication Date: 2020-06-26
QINGDAO ACAD OF AGRI SCI
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[0005] The object of the present invention is to provide a rootless rapid transplanting method for apple rootstock tissue culture seedlings, in order to solve the problems of long rooting time and low transplanting survival rate in the prior art
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Embodiment 1
[0026] Embodiment 1 A kind of apple rootstock tissue culture seedling rootless rapid transplanting method comprises the steps:
[0027] ①Induction culture: At the beginning of March in Qingdao area, the apple rootstocks planted in the glass greenhouse have germinated branches, sterilized in the ultra-clean workbench, and then inoculated on the induction medium to induce the germination of axillary buds or terminal buds. The room temperature is 25°C, and the light intensity is 2000lux. Cultivate for about 20 days to obtain aseptic buds;
[0028] ②Proliferation culture: In the ultra-clean workbench, transfer the aseptic buds of apple rootstock to the proliferation medium, at a room temperature of 25°C, with a light intensity of 2000lux, and cultivate them in tissue culture bottles for about 25 days;
[0029] ③Rooting induction: In the ultra-clean workbench, cut the plants with a height of about 2cm, transfer them to the rooting induction medium (1 / 2MS+IBA2.0mg / L+sucrose 30g / L+ag...
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The invention discloses a rootless rapid transplanting method of apple stock tissue culture seedlings. The method comprises the steps of conducting germination culture and multiplication culture on germinating branches of apple stocks, cutting plants with the height about 2 cm, transferring the plants into a rooting induction culture medium for 4 d of dark induction culture under the condition of25 DEG C, and then quickly transferring the plants into a peat medium for shading culture; removing a shading net one week after transplanting, gradually lifting plastic film to achieve ventilation and lower the environment humidity, and removing all the moisture preservation film two weeks after transplanting; when 3-4 new leaves send forth from each plant 18-22 days after transplanting, completing transplanting of the tissue culture seedlings. According to the rootless rapid transplanting method, direct cuttage is conducted 4 d after 1 / 2 MS+IBA2.0 mg / L dark induction culture is conducted, the survival rate is maintained at 80% or above, at least 25 days or so can be saved, and the efficiency of transplanting the apple stock tissue culture seedlings is greatly improved.
Description
technical field [0001] The invention relates to the technical field of tissue culture, in particular to a rootless rapid transplanting method for apple rootstock tissue culture seedlings. Background technique [0002] Excellent apple rootstock is the basis for high-quality and high-yield apples. Tissue culture technology can effectively reduce apple diseases, and supply a large number of high-quality seedlings in a short time to meet market demand. The technology of tissue culture of Malus is relatively difficult, which affects the application of tissue culture seedlings in production and promotion. High-efficiency in vitro regeneration and rooting of tissue culture seedlings are key steps in tissue culture rapid propagation technology. Whether or not rooting and the quality of rooting directly affect the emergence of seedlings. [0003] The rooting of tissue cultured seedlings is generally carried out by cuttings. Ordinary cuttings generally cut off the tissue cultured se...
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IPC IPC(8): A01H4/00A01G31/00A01G24/28
Inventor 马荣群沙广利黄粤宋正旭葛红娟张蕊芬傅景敏孙洪涛崔潇万述伟张海峰
Owner QINGDAO ACAD OF AGRI SCI