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Method for producing and separating lipids

A lipid and separator technology, applied in the production of sophorolipids, the field of equipment for the production, can solve the problems of increasing the viscosity of the medium, reducing the oxygen mass transfer coefficient, and the inhomogeneity of the fermenter

Active Publication Date: 2019-05-21
HOLIFERM IP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With known production methods, the presence of a separated sophorolipid phase in the fermenter significantly reduces the oxygen mass transfer coefficient kLa due to mass transfer resistance between the gas / liquid interface and increased medium viscosity, which leads to oxygen limitation, agitation Increased power requirements and inhomogeneities in the fermenter

Method used

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  • Method for producing and separating lipids
  • Method for producing and separating lipids
  • Method for producing and separating lipids

Examples

Experimental program
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Effect test

Embodiment 1

[0270] For Example 1, the productivity of the maximum volume is 0.69g L -1 h -1 , for Comparative Example 1C, it was 0.62g L -1 h -1 , showed an 11% increase in effective productivity when separation was used. This is due to the fact that the maximum volume achieved when separation is used is 1540 mL, which is reduced from the maximum volume of 1720 mL without separation. Due to the difference in the feed rate of the fermentation, the corresponding productivity of Example 2 was 0.57 g L -1 h -1 Not directly comparable.

[0271] The use of separation in continuous mode from early in the fermentation made it possible to control the fermentation volume to about 1.3 times the initial volume (whereas without control the fermentation volume would rise to about 1.7 times the initial volume after 280 h). This can equate to an effective productivity increase of over 30%.

[0272] In Example 2, the sophorolipid was extracted for the first time at 71.5 hours, at this time, it was ...

Embodiment 3

[0277] Example 3 uses an alternative separator and fermentation conditions. The separator used is Figure 7 Built-in settling column shown in .

[0278] Separator 1100 includes a separation chamber 1110 . In use, at the first lower end 1120 of the separator 1100, the separation chamber 1110 has a frustoconical portion 1111. The separation chamber 1110 also includes a cylindrical portion 1112 having a diameter of 50 mm and a length of 150 mm. In use, the bottom 1113 of the cylindrical portion forms the in-use upper end 1130 of the separator.

[0279] The separator 1100 includes a culture fluid inlet 1140 and has an opening to the separation chamber 1110 at the first end 1120 of the separation chamber 1110 and on the longitudinal axis A-A of the separation chamber 1110 . The inlet receives the broth 300 containing the lipid product from the fermenter 200 .

[0280] Separators include as Figure 7 The first exit (IIIA) shown in 1160 and Figure 7 The second exit (IIIB) sho...

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Abstract

There are provided methods for the production of lipids such as sophorolipids. Also provided are apparatus for use in said production.

Description

technical field [0001] The present invention relates to the production of lipids, in particular (but not exclusively) to the production of sophorolipids; the invention also relates to equipment for said production. Background technique [0002] Microbial production of lipids, and in particular glycolipids such as sophorolipids, is known. Sophorolipids consist of a hydrophilic sophorose disaccharide bound to a hydrophobic fatty acid with a typical chain length of 16-18 carbon atoms. A fatty acid can be attached to a second glucose monomer via an ester bond, yielding a lactone sophorolipid, or bound to only one glucose monomer, yielding an acidic sophorolipid due to the unbound fatty acid. These and other differences in the acetylation of the fatty acid chains and of the sophorose molecule yield a range of different structures and properties. [0003] Although several yeast strains are capable of synthesizing sophorolipids, most industrial use has focused on Candida bombicol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12P19/62C12P19/12B01D17/02C12M1/26C12M1/00
CPCB01D17/0214C12P19/12C12P19/44C12P19/62C12M33/22C12M47/10Y02E50/10C12P7/64C12P7/6409
Inventor J·B·温特伯恩B·M·多尔曼
Owner HOLIFERM IP LTD
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