Preparation method of flower-like silver nanozyme with peroxidase activity

A peroxidase, silver nanotechnology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as affecting enzyme activity, harsh synthesis conditions, and cumbersome experimental operations. Achieving high peroxidase activity, meeting practical application needs, and simple experimental operation

Active Publication Date: 2019-05-31
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these synthetic methods all use polymers or surfactants, which easily occupy active sites, thereby affecting enzyme activity, and there are problems such as uncontrollable flower branch length and narrow application range of nanozymes (such as pH, temperature).
In addition, in the first work, the nanozyme showed a certain peroxidase activity under acidic conditions, but the activity decreased significantly under neutral conditions (many enzyme-catalyzed reactions and detection applications must be carried out under neutral conditions. ); the latter two have the limitations of long synthesis process, cumbersome experimental operation, and harsh synthesis conditions.
Until now, it has been a challenge to prepare more universal flower-like silver nanozymes with peroxidase activity using simple experimental methods

Method used

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  • Preparation method of flower-like silver nanozyme with peroxidase activity
  • Preparation method of flower-like silver nanozyme with peroxidase activity
  • Preparation method of flower-like silver nanozyme with peroxidase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: regulate the branch length of silver nanoflower by changing pH

[0043] Use 0.2M NaOH solution to adjust the pH of the histidine solution to 11.2, 11.4, 11.6, and 11.8, respectively, take 9.8125 mL (containing 5 μmol of histidine), and add 62.5 μL of 40 mM silver nitrate solution to it under stirring at 500 rpm, and stir After uniformity, add 125μL of 40mM hydroxylamine solution, the final total volume of the solution is 10mL, the concentrations of histidine, silver nitrate, and hydroxylamine in the solution are 0.5mM, 0.25mM, and 0.5mM respectively, and the reaction is stopped after continuing to stir for 5min. Silver nanozymes with a diameter of 120nm and branch lengths of 9nm, 11nm, 20nm, and 23nm, respectively, the TEM images are as follows figure 1 , 2 , 3, 4, the UV-Vis absorption spectrum is shown as Figure 5 shown.

[0044] It can be seen from this example that the present invention can regulate the branch length of the silver nanozyme by chang...

Embodiment 2

[0045] Embodiment 2: adjust silver nanoflower size by changing histidine and silver nitrate molar ratio

[0046] Keep the pH in Example 1 at 11.8, control the final concentration of histidine in the mixed solution to be 0.2mM, 0.375mM, and 0.625mM respectively, and keep other conditions unchanged. After the reaction, silver particles with sizes of 90nm, 126nm, and 145nm were obtained respectively. Nanozymes, TEM images were as Image 6 , 7 , 8, and the UV-Vis absorption spectrum is shown in 9.

Embodiment 3

[0047] Embodiment 3: the peroxidase activity characterization of silver nanozyme

[0048] Take 200 μL of silver nanozyme synthesized under the conditions of pH=11.8, final histidine concentration of 0.2 mM, and mix with hydrogen peroxide and different substrate TMB (3,3',5,5'-tetramethylbenzidine) , OPD (o-phenylenediamine) or ABTS (2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid) in a Britton-Robinson buffer solution at pH = 6 was mixed to make a total volume of 3 mL . Wherein the concentration of TMB, OPD or ABTS is 0.8 mM, the buffer solution concentration is 10 mM, and the hydrogen peroxide concentration is 600 mM. After reacting for several minutes, measure the ultraviolet-visible absorption spectrum. Spectrum such as Figure 10 As shown, the comparison photos before and after the reaction are shown as Figure 11 shown.

[0049] Get pH=11.8, the silver nanozyme 200 μ L that the final concentration of histidine is synthesized under the condition of 0.2mM, and ...

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Abstract

The invention discloses a preparation method of a flower-like silver nanozyme with peroxidase activity and belongs to the technical field of preparation of nano materials. Under the condition of continuous stirring, an aqueous solution of histidine with pH of 11.2-11.8 is mixed with a silver nitrate solution, and then a reducing agent, a hydroxylamine solution is added; and after the reaction lasts for 5 minutes, the flower-like silver nanozyme with good monodispersity is obtained. By changing the pH value of the histidine, the flower branch length of the product can be adjusted, and by changing the final concentration of the histidine, the size of the product can be adjusted. The preparation method is easy to operate; by changing the reaction conditions, the flower-like silver nanozyme ofdifferent sizes and different flower branch lengths can be prepared; and the prepared flower-like silver nanozyme has good monodispersity and extremely-high peroxidase activity, and can better meet the needs of practical application.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to a method for preparing monodisperse, flower-like silver nanozyme with peroxidase activity in one step. technical background [0002] In recent years, studies have found that some metal nanoparticles have biological enzyme activity under specific reaction conditions, so they are called nanozymes (Chem. Soc. Rev. 2013, 42, 6060-6093). Compared with natural enzymes, nanozymes have attracted more and more attention due to their advantages of low production cost, good stability, high yield and recyclable use. Common metal nanozymes include Au, Ag, Pt, Pd and their various metal composite particles. Among them, silver nanozyme has excellent surface-enhanced Raman effect (Analyst 2017, 142, 2484), so it is more conducive to the analysis and activation of trace molecules. In addition, its price is relatively low, which is more conducive to industrial product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B01J23/50B01J35/00B01J35/02B82Y30/00B82Y40/00
Inventor 张丽娟韩延东张小玉杨文胜
Owner JILIN UNIV
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