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Menstrual blood preservation solution and method for cryopreservation of menstrual blood-derived mesenchymal stem cells

A technique of stem cells and cryopreservation method, which is applied in the field of menstrual blood preservation solution and cryopreservation of menstrual blood-derived mesenchymal stem cells, can solve the problems of high price and high toxicity, and achieves the maintenance of cell viability, high cell viability rate, and cell viability. Good results

Active Publication Date: 2021-11-23
陕西医赛尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amphotericin B, a broad-spectrum antifungal agent, is highly toxic to cells and cannot be directly used for cell culture. Water-soluble amphotericin B specially used for cell culture is mostly foreign products, and the price is relatively expensive.

Method used

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  • Menstrual blood preservation solution and method for cryopreservation of menstrual blood-derived mesenchymal stem cells
  • Menstrual blood preservation solution and method for cryopreservation of menstrual blood-derived mesenchymal stem cells
  • Menstrual blood preservation solution and method for cryopreservation of menstrual blood-derived mesenchymal stem cells

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Experimental program
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Effect test

Embodiment 1

[0031] Such as Figure 1-Figure 6 As shown, the menstrual blood preservation solution includes:

[0032] The base solution of the preservation solution is 0.9% physiological saline, and the preservation solution also includes penicillin, streptomycin and fluconazole added to the base solution;

[0033] The final concentrations of penicillin and streptomycin are 100 U / mL, and the final concentration of fluconazole is 0.5% by mass to volume.

[0034] No anticoagulant is added to the menstrual blood preservation solution, so as to avoid the damage of the anticoagulant to the cells and ensure the activity of the cells in the menstrual blood sample to the greatest extent. At the same time, antibiotics are added to the menstrual blood preservation solution to inhibit the growth of vaginal bacteria and fungi, and the collection should be aseptic as possible to reduce the chance of bacterial and fungal contamination from the source.

[0035] In the method for cryopreserving menstrua...

Embodiment 2

[0046] Menstrual blood preservation solution, including:

[0047] The base solution of the preservation solution is 0.9% physiological saline, and the preservation solution also includes penicillin, streptomycin and fluconazole added to the base solution;

[0048] The final concentration of penicillin and streptomycin is 200 U / mL, and the final concentration of fluconazole is 0.75% by mass to volume.

[0049] No anticoagulant is added to the menstrual blood preservation solution, so as to avoid the damage of the anticoagulant to the cells and ensure the activity of the cells in the menstrual blood sample to the greatest extent. At the same time, antibiotics are added to the menstrual blood preservation solution to inhibit the growth of vaginal bacteria and fungi, and the collection should be aseptic as possible to reduce the chance of bacterial and fungal contamination from the source.

[0050] In the method for cryopreserving menstrual blood-derived mesenchymal stem cells in...

Embodiment 3

[0061] Menstrual blood preservation solution, including:

[0062] The base solution of the preservation solution is 0.9% physiological saline, and the preservation solution also includes penicillin, streptomycin and fluconazole added to the base solution;

[0063] The final concentrations of penicillin and streptomycin are 300 U / mL, and the final concentration of fluconazole is 1% by mass to volume.

[0064] No anticoagulant is added to the menstrual blood preservation solution, so as to avoid the damage of the anticoagulant to the cells and ensure the activity of the cells in the menstrual blood sample to the greatest extent. At the same time, antibiotics are added to the menstrual blood preservation solution to inhibit the growth of vaginal bacteria and fungi, and the collection should be aseptic as possible to reduce the chance of bacterial and fungal contamination from the source.

[0065] In the method for cryopreserving menstrual blood-derived mesenchymal stem cells in ...

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Abstract

A menstrual blood preservation solution and a method for cryopreservation of menstrual blood-derived mesenchymal stem cells, comprising: the base solution of the preservation solution is 0.9% physiological saline, and the preservation solution also includes penicillin, streptomycin and fluoride added to the base solution Conazole; wherein the final concentration of penicillin and streptomycin is 100U / mL-300U / mL, and the final concentration of fluconazole is 0.5%-1% by mass to volume. The final concentration of penicillin and streptomycin was 100 U / mL. Combined with its menstrual blood source mesenchymal stem cell cryopreservation method, clean and disinfect the female vulva when collecting women's menstrual blood, use an autoclaved menstrual cup to collect menstrual blood, store it in a preservation solution, reduce the chance of contamination from the source of collection, and preserve the cells in menstrual blood Vitality: The mesenchymal stem cell seed cells obtained by separating the collected menstrual blood have good cell activity after cryopreservation and resuscitation, the primary cultured cells have high proliferation activity, the subcultured cells have high viability and low pollution rate.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a menstrual blood preservation solution and a method for freezing mesenchymal stem cells derived from menstrual blood. Background technique [0002] Menstrual blood-derived mesenchymal stem cells are stem cells isolated and cultured from women's menstrual blood. The earliest research team led by American Musina obtained it from the menstrual blood of healthy women. In 2008, a research team led by Hida in Japan also successfully isolated and cultured this kind of stem cells with multiple differentiation functions using women's menstrual blood. Among known human cells, menstrual blood-derived mesenchymal stem cells are stem cells with the strongest self-replication and directional differentiation capabilities. They originate from the endometrium and are excreted through menstrual blood. Its differentiation potential is similar to that of embryonic stem cells, and it has the ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 谢晓燕张小波杨劲鹏
Owner 陕西医赛尔生物科技有限公司