Hydrogel nanoparticles for measuring ionizing radiation dose and method of preparation thereof
An ionizing radiation and nanoparticle technology, which is used in measurement devices, radiation measurement, chemical instruments and methods, etc., can solve the problems of large measurement deviation, easy diffusion of ions, and time-consuming reading, achieving good stability and solving low sensitivity. , the effect of low error rate
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Embodiment 1
[0057] The preparation of embodiment 1 hydrogel nanoparticles
[0058] The present embodiment provides a kind of hydrogel nanoparticle and preparation method thereof based on colorimetric fluorescence method for measuring ionizing radiation dose, comprising the following steps:
[0059] 1. Dissolve 0.5 mg APMA and 1 mg TAMRA-SE in 250 μL of sodium borate buffer (pH=9.5), stir at room temperature in the dark for 24 hours to obtain APMA-modified TAMRA (hereinafter referred to as TAMRA-APMA), and store at 4°C for later use. Its reaction schematic diagram is as follows figure 1 shown.
[0060] 2. Dissolve 300mg of AAm, 180mg of BIS and 300mg of APMA in 2mL of deionized water, then add to 250μL of TAMRA-APMA prepared in step 1, and filter the mixture with a 0.2μm filter to obtain a reaction mixture.
[0061] 3. Add 3.2mL of surfactant Brij 30 and 1.59g of AOT into 43mL of n-hexane and stir to dissolve. At the same time, add 2mL of the reaction mixture prepared in step 1, N 2 Sti...
Embodiment 2
[0066] The preparation of embodiment 2 hydrogel nanoparticles
[0067] The present embodiment provides a kind of hydrogel nanoparticle and preparation method thereof based on colorimetric fluorescence method for measuring ionizing radiation dose, comprising the following steps:
[0068] 1. Dissolve 1.25 mg of rhodamine b-glucan (RITC-Dextran), 300 mg of AAm, 180 mg of BIS and 300 mg of APMA in 2 mL of deionized water, and then filter with a 0.2 μm filter to obtain a reaction mixture. Rhodamine b-glucan in this step can also be replaced by Texas Red-glucan or rhodamine b-isothiocyanate.
[0069] 2. Add 3.2mL of surfactant Brij 30 and 1.59g of AOT into 43mL of n-hexane, stir and dissolve, and add 2mL of the reaction mixture prepared in step 1 at the same time, N 2 Stir at high speed under the atmosphere for 20min, then add 80μL of 10% APS and 60μL of tetramethylethylenediamine, N 2 The reaction was carried out for 2 h under the atmosphere to obtain PAA-based hydrogel nanoparti...
Embodiment 3
[0073] The preparation of embodiment 3 hydrogel nanoparticles
[0074] Hydrogel nanoparticles for measuring ionizing radiation dose based on colorimetric fluorescence method were prepared according to the method of Example 1, except that AAm in step 2 could be replaced by hydroxyethyl methacrylate of equimolar mass.
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