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A lysine detection method based on quantum dots and gold nanoparticles

A detection method, lysine technology, applied in the field of analysis and detection, can solve the problems of cumbersome detection process and poor specificity of lysine detection technology, and achieve the effects of simple detection method, poor response sensitivity and simple synthesis process

Active Publication Date: 2019-12-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a lysine detection method based on quantum dots and nano-gold for the problems of poor specificity and cumbersome detection process of the existing lysine detection technology, which is based on the high specificity of nano-gold and lysine Sexual response, and using quantum dots as fluorescent sensing, combining nanomaterials with sensing technology to construct a reversible fluorescent sensor, which can show excellent selectivity and sensitivity to lysine, and the detection method involved is simple, Suitable for promoting applications

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  • A lysine detection method based on quantum dots and gold nanoparticles
  • A lysine detection method based on quantum dots and gold nanoparticles
  • A lysine detection method based on quantum dots and gold nanoparticles

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Embodiment 1

[0039] A kind of lysine detection method (feasibility study) based on quantum dot and nano gold, comprises the steps:

[0040] 1) Take 100 μL of 552 nmol / L cadmium telluride quantum dot solution, add 900 μL double distilled water and measure its fluorescence intensity at 630 nm at an excitation wavelength of 360 nm, and record it as F (results in figure 1 A);

[0041] 2) Take 100 μL of cadmium telluride quantum dot solution of 552nmol / L, 90 μL of modified nano-gold solution of 47.2nmol / L, and 810 μL of double-distilled water, measure its fluorescence intensity at 630nm at an excitation wavelength of 360nm after mixing, and record as f 0 (results see figure 1 C);

[0042] 3) Take 100 μL of 552nmol / L cadmium telluride quantum dot solution, 90 μL of 47.2nmol / L modified nano-gold solution, 100 μL of 1mmol / L lysine solution and 710 μL of double distilled water, mix and measure at 360nm excitation wavelength Its fluorescence intensity at 630nm is recorded as F 1 (results see f...

Embodiment 2

[0045]A kind of lysine detection method (high selectivity research) based on quantum dot and nano gold, comprises the steps:

[0046] 1) Take 100 μL of cadmium telluride quantum dot solution of 552nmol / L, 90 μL of modified nano-gold solution of 47.2nmol / L, and 810 μL of double-distilled water, measure its fluorescence intensity at 630nm at an excitation wavelength of 360nm after mixing, and record as f 0 ;

[0047] 2) Take 100 μL of 552nmol / L cadmium telluride quantum dot solution, 90 μL of 47.2nmol / L modified nano-gold solution, and 100 μL of 13 kinds of amino acid solutions (lysine 100 μmol / L; serine, histidine, leucine , proline, serine, aspartic acid, threonine, arginine, phenylalanine, glutamic acid, isoleucine, methionine concentration is 1000μmol / L), 710μL double distilled water, After mixing, measure its fluorescence intensity at 630nm at an excitation wavelength of 360nm, and record it as F 1 ;

[0048] 3) Calculate the change in fluorescence intensity after addin...

Embodiment 3

[0051] A kind of lysine detection method (specificity research) based on quantum dot and nano gold, comprises the steps:

[0052] 1) Take 100 μL of cadmium telluride quantum dot solution of 552nmol / L, 90 μL of modified nano-gold solution of 47.2nmol / L, and 810 μL of double-distilled water, measure its fluorescence intensity at 630nm at an excitation wavelength of 360nm after mixing, and record as f 0 ;

[0053] 2) Take 552nmol / L cadmium telluride quantum dot solution 100μL, 47.2nmol / L modified nano-gold solution 90μL, 100μL lysine solution (2,6-diaminocaproic acid) A and compared with lysine Lack of carboxyl, α-NH 2 and ε-NH 2 1,5-diaminovaleric acid solution (B), 6-aminocaproic acid solution (C) and DL-norleucine solution (D), then add 710μL double distilled water, mix and measure at 360nm excitation wavelength Its fluorescence intensity at 630nm is recorded as F 1 ;

[0054] 3) Calculate the change in fluorescence intensity after adding different amino acid solutions (...

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Abstract

The invention provides a lysine detecting method based on quantum dots and nano gold, wherein the reversible fluorescence method has the advantages of high selectivity and sensitive. The fluorescenceintensity change of cadmium quantum dots and a modified nano gold mixed system before and after different concentrations of lysine are adding is determined, and the linear relationship with the concentration of lysine is established to detect the content of lysine mainly including fluorescent quantum dots. According to the invention, the fluorescent quantum dots used are cadmium telluride quantumdots; the nano gold is dodecyltrimethylammonium bromide self-assembled citrate-terminated nano gold; a new reversible fluorescent probe is formed; the reversible fluorescent probe can detect the lysine content efficiently, quickly and accurately; the detection range is 0.5 to 100 mu mol / L; the detection limit is up to 3.22nmol / L; a synthesis process involved is simple and the analysis cost is low;and the selectivity for lysine detection can be significantly improved.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a lysine detection method based on quantum dots and nano gold. Background technique [0002] Lysine is one of the eight essential amino acids in the human body, which plays a vital role in the metabolic process in the human body. As an important intermediate in the metabolic pathway, lysine can promote human development, enhance immune function, and play a role in improving the central nervous system. The role of nervous tissue function. However, abnormal lysine metabolism will lead to some physiological diseases, such as hypersarcosine disease, blood or cystinuria and so on. [0003] At present, many sensors for the detection of lysine have been tried to be developed, such as sensor systems based on carbon dots, nano-gold, Thioflavin-T-heparin, etc. Although these sensors can quickly realize the analysis and detection of lysine, but at the same time ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 杨小龙时琼付海燕杨天鸣
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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