Blood agglutination determination method and device

Abstract

A blood agglutination determination method and device are provided. The method comprises steps of placing blood cells or serums having a known blood type in the specimen pores of a micropore plate, and placing the blood cells or serum of the detected blood in each specimen pore to form a reaction sample; scanning the reaction sample in the specimen pores to obtain the absorbance value of a light absorption point, defining an effective light absorption point range by using a middle light absorption point as a center, and eliminating the light absorption points with errors at the edges; selecting the minimum absorbance value and the maximum absorbance value within the effective light absorption point range; using the minimum absorbance value as a base number, obtaining a difference value between the maximum absorbance value and the minimum absorbance value, calculating a ratio of the difference value to the minimum absorbance value as the base number; presetting an agglutination reference value, and comparing the ratio with the agglutination reference value, determining that the blood in the specimen pores is agglutinated when the ratio is greater than the agglutination reference value, or determining that the blood in the specimen pores is not agglutinated when the ratio is less than or equal to the agglutination reference value. The method can quickly determine whether the mixed sample is agglutinated, and has high accuracy, and a wide application range.

Description

technical field [0001] The invention relates to a blood coagulation judging method and device, and relates to the technical field of blood processing. Background technique [0002] As we all know, blood agglutination is a serum immune reaction that may occur when blood of different blood types meets. It can be seen under a microscope that blood cells in the blood will adhere to each other and form a group. The microplate reader has the highest penetration rate in various blood stations, hospitals and various blood laboratories, and the microplate reader has a scanning function. [0003] At present, there are several methods for judging blood coagulation: one is the image analysis method, which judges whether the blood has coagulated by capturing images and analyzing the images. instrument. The other is to judge by naked eyes, such as ABO blood type test card. The first way, using large-scale equipment, cannot be used due to high cost and low penetration rate; the second w...

AI Technical Summary

Problems solved by technology

The first method uses large-scale equipment, which cannot be used due to high cost and low penetration rate; the second method uses manual visual inspection, which has low detection efficiency and poor accuracy of detection results

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