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A method for producing protein feed and feed product

A protein feed and seed technology, applied in animal feed, animal feed, food processing, etc., can solve problems such as difficult animal utilization, achieve good palatability, reduce costs, and solve environmental pollution effects

Active Publication Date: 2020-12-29
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the protein content of hydrolyzed feather meal is very high, usually above 80%, 85% to 90% of it is keratin, which belongs to hard protein, and the structure of peptides is composed of disulfide bonds (-S-S-) and sulfur It is connected by hydrogen bonds and has great stability. It is insoluble in water, dilute acid and salt solution, and it is difficult to be used by animals without processing.

Method used

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  • A method for producing protein feed and feed product
  • A method for producing protein feed and feed product
  • A method for producing protein feed and feed product

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A method for preparing a protein, comprising the steps of:

[0039] S1. Preparation of liquid strains

[0040] (1) For slant-plane strain cultivation, configure YPD medium with the following weight percentages: 1% yeast extract powder, 2% peptone, 2% glucose, sterilized at 115° C. for 20 minutes, and add 1.5% agar powder to make slant medium;

[0041] (2) select Pichia pastoris as the bacterial classification of fermenting and producing enzyme;

[0042] (3) Inoculate the above-mentioned strains on the slant, culture them in an incubator at 28°C for 2 days, then transfer them to YPD liquid medium at 30°C and 250 rpm for 24 hours, and transfer them again (inoculation amount is 2%), at 30°C , 250r / min cultured for 24h, prepared into a liquid seed solution.

[0043] S2, preparation of keratinase crude enzyme liquid:

[0044] (1) Thalline growth stage: configure BSM medium by the following weight percentage: phosphoric acid 2.27%, CaSO 4 0.093%, K 2 SO 4 1.82%, MgSO ...

Embodiment 2

[0053] The difference between this embodiment and Example 1 is that the fermentation substrate in step S3 (1) is a mixture of feather meal and soybean meal, and the raw material is prepared with a mass ratio of feather meal: soybean meal=1:2; the rest of the steps are With embodiment 1, obtain protein feed.

Embodiment 3

[0055] The difference between this embodiment and Example 1 is that the fermentation substrate in step S3 (1) is a mixture of feather meal and soybean meal, and the raw material is prepared with a mass ratio of feather meal: soybean meal=1:3 to prepare the fermentation substrate; With embodiment 1, obtain protein feed.

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Abstract

The invention discloses a method for producing high-grade protein feed and a feed product. Feather meal and bean pulp mixture serves as a main raw material, proportioning is optimized, crude enzymes are attached to perform solid fermentation, finally, elaborate processing is performed to obtain the high-grade protein feed, and the protein content of the obtained high-grade protein feed product ishigher than or equal to 60%. Huge-yield by-products in poultry breeding industry are sufficiently used, environmental pollution is reduced to a great extent, and the high-protein-content feed producedby a microbiologic fermentation technique is good in quality, easy to absorb, short in fermentation period and simple in technological operation.

Description

technical field [0001] The invention belongs to the technical field of feed production, and in particular relates to a method for producing protein feed and a feed product. Background technique [0002] Today, with the rapid development of breeding and animal husbandry, the shortage of animal protein sources has gradually become a global problem, especially in my country. At present, there are mainly two kinds of protein feeds used in animal diets: one is animal or insect protein such as common fish meal, meat and bone meal, silkworm chrysalis, which is rich in nutrition and balanced in amino acid nutrition, which is more conducive to animal digestion and absorption. The potency is higher than other plant proteins such as soybean meal, rapeseed meal and peanut meal, etc., but its disadvantage is that it is expensive, mainly depends on imports, and domestic resources are in short supply. In addition, the potential threat of diseases such as mad cow disease has led to ruminant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/26A23K10/37A23K50/30A23K50/80A23K50/00
CPCA23K10/12A23K10/26A23K10/37A23K50/00A23K50/30A23K50/80Y02P60/87Y02A40/818
Inventor 彭楠刘云常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV