Endophytic fungus Onygenales X117 of incarvillea younghusbandii sprague, and preparation method and application of fermented product of endophytic fungus Onygenales X117
A technology for Zangbo flower and endophytic fungi, applied in the biological field, can solve the problems of large-scale synthesis and application of unfavorable compounds, no significant activity of derivatives thereof, complicated steps, etc., and achieves good application prospects and good anti-inflammatory activity. , the effect of broad development space
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0056] Example 1 Isolation and Identification of the Endophytic Fungus Onygenales X117 from Zangboluohua
[0057] 1. Isolation of strains
[0058] Sample: The root of a Tibetan medicine Incarvillea younghus bandii Sprague in the saline-alkali land beside the Bamucuo Salt Lake in Bangor County, Tibet Autonomous Region.
[0059] Isolation method: Sterilize the root of the fresh Tibetan medicinal material Zangboluo flower, cut off the outer root bark, inoculate it into PDA medium, Martin medium or Chase medium under aseptic conditions, and culture it below 20°C for 5-20 days , to obtain a single strain Onygenales X117; the obtained strain Onygenales X117 was preserved at 4° C. on a common PDA medium slant.
[0060] 2. Identification of strains
[0061] The biological characteristics of this strain are that when cultured at a constant temperature of 5-15°C on PDA medium, the surface of the colony is orange short hairy hyphae, and the back is orange-red with spores; when cultured...
Embodiment 2
[0065] Example 2 Fermentation of the endophytic fungus Onygenales X117 and the extraction, separation and purification of the compound Gymnoascolide A
[0066] 1. Experimental method
[0067] S1. Potato residue solid medium was used, and the bacterial strain of the endophytic fungus Onygenales X117 strain of Tibetan medicinal material was inserted into a triangular flask filled with potato residue solid medium, and cultured statically for 30 days under variable temperature conditions of 10-28°C;
[0068] S2. Add methanol-chloroform (v / v 1:1) mixed solvent into the conical flask, extract 4 times, and concentrate the extract by rotary evaporation. Ethyl ester-petroleum ether system (1:20, 1:10, 1:5, 1:4, 1:3, 1:2, 1:1) was eluted, and the obtained 1:20 components were purified by petroleum The compound Gymnoascolide A was obtained by recrystallization from ether-ethyl acetate.
[0069] 2. Experimental results
[0070] The productive rate of compound Gymnoascolide A obtained i...
Embodiment 3
[0071] Example 3 Fermentation of the endophytic fungus Onygenales X117 and the extraction, separation and purification of the compound Gymnoascolide A
[0072] 1. Experimental method
[0073] S1. Potato residue solid medium was used, and the bacterial strain of the endophytic fungus Onygenales X117 strain of Tibetan medicinal material was inserted into a triangular flask filled with potato residue solid medium, and cultured statically for 25 days under variable temperature conditions of 5-25°C;
[0074] S2. Add methanol-chloroform (v / v 1:1) mixed solvent into the conical flask, extract 4 times, and concentrate the extract by rotary evaporation. Ethyl ester-petroleum ether system (1:20, 1:10, 1:5, 1:4, 1:3, 1:2, 1:1) was eluted, and the obtained 1:20 components were purified by petroleum The compound Gymnoascolide A was obtained by recrystallization from ether-ethyl acetate.
[0075] 2. Experimental results
[0076] The yield of the compound Gymnoascolide A obtained in this ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More