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Preparing method of antibacterial active substances of bacillus pumilus and application
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A technology of Bacillus pumilus and antibacterial active substances, which is applied in the field of microbial technology and application, can solve problems such as the research of active substances of Bacillus pumilus, and achieve the effects of stable products, short production cycle, and inhibition of pathogenic bacteria
Active Publication Date: 2019-07-12
BIOLOGY INST OF SHANDONG ACAD OF SCI
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[0010] The focus of the above technologies is mostly on the prevention and control of plant diseases and the promotion of plant growth, and there are no reports on further research on the active substances of Bacillus pumilus
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Embodiment 1
[0070] A strain of Bacillus pumilus BP, which was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short) on March 21, 2019, address: Courtyard 3, No. 1, Beichen West Road, Chaoyang District, Beijing No., Institute of Microbiology, Chinese Academy of Sciences), the deposit number is CGMCC NO.17424.
[0071] The biological characteristics of the bacterial strain: it is of the genus Bacillus, the thalline is thin rod-shaped, and Gram-positive. It grows transparent on the LB slope and can produce antibacterial active ingredients.
Embodiment 2
[0073] The culture method of bacillus pumilus short (Bacillus pumilus) BP, the steps are as follows:
[0074] (1) Inoculate the above-mentioned Bacillus pumilus into the seed culture medium, and culture it for 16 hours under the conditions of 37° C. and 180 rpm to obtain the seed liquid;
[0075] Described seed culture medium is LB broth culture medium, and every liter of component is as follows:
[0076] 10 grams of peptone, 10 grams of sodiumchloride, 5 grams of yeast extract, and water to 1000 ml;
[0077] (2) Inoculate the seed solution prepared in step (1) into the fermentation medium at a ratio of 3% by volume, and cultivate it at 30° C. at 180 rpm for 48 hours to obtain a fermentation solution;
[0078] Described fermentation medium, every liter of component is as follows:
[0079] Glucose 30.0g, K 2 HPO 4 ×3H 2 O 7.0g, KH 2 PO 4 3.0g, (NH 4 ) 2 SO 4 1.5g, sodium citrate 3H 2 O0.5g, MgSO 4 ·7H 2 O 0.1g, pH7.0~7.2, add water to make up to 1L.
Embodiment 3
[0081] The preparation method of the antibacterial active substance derived from bacillus pumilus (Bacillus pumilus) BP, the steps are as follows:
[0082] (i) The fermented liquid prepared in Example 2 was centrifuged at 3500 rpm for 35 minutes, the supernatant was taken, and concentrated to 8 times the concentration of the original solution by rotary evaporation to obtain a concentrated solution;
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Abstract
The invention relates to a preparing method of antibacterial active substances of bacillus pumilus and application. The strain of the bacillus pumilus (BP) is preserved in China General Microbiological Culture Collection Center in March 21, 2019, wherein the China General Microbiological Culture Collection Center is located in the Institute of Microbiology of Chinese Academy of Sciences, in Building No.3, Yard No.1, Road Beicheng West, Chaoyang District, Beijing, and the preservation number is CGMCC NO.17424. The invention further relates to the antibacterial active substances prepared by means of the strain and the relevant application. According to the preparing method of the antibacterial active substances of the bacillus pumilus and the application, three fatty alcohol compounds with the antibacterial activity are extracted from the bacillus pumilus for the first time, while the three fatty alcohol compounds are not found in existing bacillus pumilus.
Description
technical field [0001] The invention relates to a preparation method and application of an antibacterial active substance of Bacillus pumilus, belonging to the technical field of microbial technology and application. Background technique [0002] At present, the extensive use of antibiotics in the livestock and poultry breeding industry has led to the emergence of drug resistance and veterinary drug residues, seriously affecting the quality of livestock products, and the excretion of residues has also caused soil and water pollution. It destroys the ecological environment and directly endangers human health. Environmental pollution has caused the deterioration of the human living environment, leading to increasingly prominent food safety issues. [0003] Antibacterial active substances are research hotspots at home and abroad in recent years, and can be widely used in biological feed, biocontrol, medicine, natural food preservatives, cosmetics, etc. Among them, Bacillus ca...
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