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Macrofungus fruiting body transplanted strain preparation method

A fungal fruiting body, large-scale technology, applied in the field of preparation of strains transferred from large fungal fruiting bodies, can solve the problems that affect the success rate of strain separation, low mycelium content, bacterial contamination, etc., and achieve high success rate and easy The effect of popularization and simple technology

Inactive Publication Date: 2019-07-19
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

However, the content of mycelium in the fruiting bodies of most macrofungi is relatively small, and they are easily contaminated by bacteria during the transfer process, which seriously affects the success rate of strain isolation.

Method used

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  • Macrofungus fruiting body transplanted strain preparation method
  • Macrofungus fruiting body transplanted strain preparation method
  • Macrofungus fruiting body transplanted strain preparation method

Examples

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specific Embodiment

[0033] Specific Example: Transfer of Boletus Fruiting Body Strains

[0034] The embodiments of the present invention will be described in further detail below in conjunction with the accompanying drawings, such as figure 1 , figure 2 Shown:

[0035] (1) Fruit body collection: In August 2018, the fruit body was collected in Yanqing Mountain, Beijing. Care should be taken to select a number of boletus fruiting bodies that are relatively tender and rolled in the edge of the cap, and gently picked off with a small amount of soil at the root. , Put it back in the portable refrigerator.

[0036] (2) Preparation of culture medium: Weigh 200 g of peeled potatoes and cut them into small pieces, boil them with an appropriate amount of distilled water, and filter with gauze after the whole process lasts for 30 minutes. Add 20g glucose, 15-20g agar, KH to the filtrate 2 PO 4 3g, MgSO 4 ·7H 2 O1.5g was heated again until completely dissolved (add water to 1000mL). Put it into a high-temperatu...

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Abstract

The invention discloses a macrofungus fruiting body transplanted strain preparation method. The method includes: firstly, bringing back wild vigorous tender macrofungus fruiting bodies in a refrigerating manner, carefully flushing with distilled water, and uniformly spraying 75% alcohol to the fruiting bodies to disinfect; then, halving from stems to caps, cutting stem and cap tissues in size of about 0.5cm*0.5cm by using a sterile sharp blade, and performing inoculation in a potato solid medium PDA containing streptomycin; finally, putting an inoculated petri dish in an incubator, culturing in dark at 25 DEG C for 7-10 days, observing mycelium germination conditions, selecting white villous mycelia (culture medium included) away from living contaminants, and performing expansion culturingin a newly prepared PDA culture medium to obtain a single strain. By adoption of the PDA containing the streptomycin and a tissue isolation method for fruiting body transplanting, the pure strain isobtained, and simplicity in operation, time saving and labor saving are realized.

Description

Technical field [0001] The invention relates to a preparation technology of strains, in particular to a preparation method for transferring strains from large fungi fruiting bodies. Background technique [0002] Macrofungi refers to a class of higher fungi that can form fleshy or gelatinous fruiting bodies, and is the most diverse group of organisms in nature. As the main decomposer in the ecosystem, it plays a decisive role in the energy and material cycle of the ecosystem. In addition, many large fungi are edible and medicinal fungi with high nutritional value and medicinal value. With the continuous deepening of modern biotechnology, the macroscopic research of the morphological characteristics and tissue structure of large fungi has gradually risen to the field of molecular biology. Therefore, the transfer of large fungal fruit bodies to obtain pure fungal strains has become a key technical link. [0003] At present, the methods for obtaining the transfer of macrofungi fruit ...

Claims

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Application Information

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IPC IPC(8): A01G18/40
CPCA01G18/40
Inventor 高广磊赵珮杉张英郭米山任悦魏晓帅
Owner BEIJING FORESTRY UNIVERSITY