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Method for preventing and controlling pathogen infection of plants

A technology for pathogens and plants, applied in the field of pesticides, can solve the problems of strong infectivity and no effective control methods for germs

Active Publication Date: 2019-07-23
NATURAL MEDICINE INST OF ZHEJIANG YANGSHENGTANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Plant diseases caused by phloem parasites, such as yellow dragon disease, zebra flake disease, grape gold disease, pear decay disease, etc., are highly contagious, and there is still no effective control method for such bacteria

Method used

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  • Method for preventing and controlling pathogen infection of plants
  • Method for preventing and controlling pathogen infection of plants
  • Method for preventing and controlling pathogen infection of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: antibacterial experiment to papaya endophyte

[0067] 1.1 Experimental equipment and reagents

[0068] 1.1.1 Experimental equipment

[0069] Microplate reader (BioTek), ultra-clean bench (Suzhou Purification Equipment Co., Ltd.), sterilizer (Sanyo), pH meter (METTLER TOLEDO), incubator (MJX-250B-Z), etc.

[0070] 1.1.2 Experimental reagents

[0071] α-ketoglutarate (Sigma), N-carbamoylmethylethanesulfonic acid (Sigma), TMN-FH insect medium (Sigma), fetal bovine serum (Gibco), papaya endophytes (Liberibacter crescens BT- 1, Shanghai Baolu Biology), etc.

[0072] 1.2 Experimental steps:

[0073] 1.2.1 Preparation of medium

[0074] The preparation method is shown in Table 1.

[0075] Table 1 Preparation of BM7 medium

[0076]

[0077] 1.2.2 Antibacterial experiment

[0078] Since phloem parasites cannot be artificially cultured in vitro so far, it is impossible to use phloem parasites to screen antibacterial drugs in vitro. At present, the method ...

Embodiment 2

[0088] Embodiment 2: Antibacterial experiment to Huanglongbing pathogenic bacteria in detached leaves

[0089] 2.1 Experimental equipment and reagents

[0090] 2.1.1 Experimental equipment

[0091] Real-time fluorescence quantitative PCR instrument (Roche), centrifuge (Beckman), ultra-clean bench (Suzhou Purification), sterilizer (Sanyo), ultra-micro nucleic acid protein analyzer (Thermo), etc.

[0092] 2.1.2 Experimental reagents

[0093] CTAB-Triton DNA extraction solution, proteinase K (10mg / mL), 1M dithiothreitol (DTT), Tris-phenol-chloroform-isoamyl alcohol mixture (the volume of Tris-phenol, chloroform, and isoamyl alcohol ratio of 25:24:1), chloroform-isoamyl alcohol mixture (the volume ratio of chloroform to isoamyl alcohol is 24:1), 3M sodium acetate, isopropanol, 75% ethanol, 10×PCR Buffer, 10mmol dNTPs, 5u / μl rTaq, etc., all of the above reagents were purchased from TaKaRa.

[0094] 2.2 Experimental method

[0095] 2.2.1 Leaf collection and administration

[0...

Embodiment 3

[0105] Embodiment 3: Antibacterial experiment to citrus Huanglongbing

[0106] experimental method

[0107] In May 2018, 210 diseased trees suspected of being infected with Huanglongbing were selected from the citrus field in Huban Village, Banshi Town, Anyuan County, Ganzhou City, Jiangxi Province, and tested for Huanglongbing. The results showed that the incidence rate of Huanglongbing was 100%. Randomly select diseased trees infected with Huanglongbing to carry out drug antibacterial test, each test group has 10 diseased trees, adopts the trunk infusion method, and inputs different concentrations of drug solutions (i.e. the aqueous solution of compound 1-10, if the drug is insoluble in water, it can be added in an appropriate amount) DMSO was dissolved, but the final concentration of DMSO was no more than 1%), and another 10 plants were randomly selected as controls (input clear water). From May 1st, the drug was applied once at the beginning of each month, and each applica...

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PUM

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Abstract

The invention relates to a compound shown in a formula I, stereoisomers of the same, or chemical acceptable salts for agriculture or forestry or use of a composition containing the compound shown in the formula I, stereoisomers of the same, or chemical acceptable salts for agriculture or forestry for preventing, controlling or treating infectious diseases of plants caused by pathogens. The invention also relates to a method for preventing, controlling or treating pathogen infection of the plants and a method for preventing, controlling or treating the infectious diseases of the plants caused by the pathogens.

Description

technical field [0001] The application belongs to the technical field of pesticides, relates to a method for preventing, controlling or treating plants infected by pathogens, and also relates to a method for preventing, controlling or treating infectious diseases of plants caused by pathogens. Background technique [0002] Phloem parasites are a type of harmful bacteria that parasitize in the phloem of plants. Since this type of bacteria mainly parasitizes in the phloem of plants, once they colonize and reproduce in the phloem, they will cause phloem tissue necrosis and sieve tube blockage, affecting the supply of nutrients in plants. Transport and absorption, and then cause disease and death of plants. Such bacteria are easily carried and infected by flying insects such as psyllids and cicadas, and can also be infected by grafting. [0003] At present, it is found that most of the devastating plant diseases in the world are related to phloem parasites, such as Huanglongbin...

Claims

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Application Information

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IPC IPC(8): A01N43/713C07D257/04A01P1/00
CPCA01N43/713C07D257/04
Inventor 张正方韩铨胡柳毛熊兴
Owner NATURAL MEDICINE INST OF ZHEJIANG YANGSHENGTANG