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Method for detecting residual amount of quizalofop-p-ethyl in phaseolus calcaratus

A technology of quizalofop-p-ethyl and detection method is applied in the field of detection of quizalofop-p-ethyl in adzuki bean, and can solve the problems of quizalofop-p-ethyl without adzuki bean, difficulty in eliminating magazine interference, expensive instruments, etc. Fast and efficient detection, rapid qualitative and quantitative analysis, and the effect of fast analysis

Pending Publication Date: 2019-07-26
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional method of detecting pesticide residues in agricultural products is either time-consuming, or the equipment is expensive and the operation is complicated. Moreover, because the ingredients in the red bean are complex, especially containing a lot of pigments, the traditional processing method is difficult to eliminate the interference of magazines, which has a great impact on the detection. impact, and there is no fast, efficient and simple detection method specifically for quizalofop-p-ethyl in Chixiaodou, therefore, there is an urgent need for a method for rapid detection of quizalofop-p-ethyl in Chixiaodou

Method used

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  • Method for detecting residual amount of quizalofop-p-ethyl in phaseolus calcaratus
  • Method for detecting residual amount of quizalofop-p-ethyl in phaseolus calcaratus
  • Method for detecting residual amount of quizalofop-p-ethyl in phaseolus calcaratus

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 is the unmodified QuEChERS-pretreatment method.

[0026] Sample pretreatment: Clean the commercially available Chixiaodou, dry it, crush it in a grinder, pass it through a 100-mesh sieve, accurately weigh 5g of Chixiaodou sample, add 5mL of acetonitrile extraction solvent, vortex for 2min, add 3g of anhydrous MgSO 4 , vortexed for 30s, then added 0.75g NaCl, vortexed for 30s, centrifuged at 4000r / min for 10min, pipetted 2.5ml of the supernatant into a test tube, concentrated to 1mL by nitrogen blowing at 45°C, transferred to a tube containing 150mg PSA and 0.9g Anhydrous MgSO 4 In a 2mL centrifuge tube, centrifuge at 10,000r / min for 2min, absorb the supernatant, pass through a 0.22μm filter membrane, and wait for detection.

Embodiment 2

[0028] Example 2 is the improved QuEChERS-pretreatment method.

[0029] Sample pretreatment: Clean the commercially available Chixiaodou, dry it, put it in a pulverizer, and pass it through a 100-mesh sieve. Accurately weigh 5g of Chixiaodou sample, add 15mL acetic acid acetonitrile extraction solvent, vortex for 2min, add 0.3g NaCl, Vortex for 30s, then add 1.5g anhydrous MgSO 4 , vortexed for 30s, centrifuged at 4000r / min for 10min, pipetted 2.5ml of the supernatant into a test tube, concentrated to 1mL by nitrogen blowing at 45°C, transferred to a 2mL centrifuge tube containing 25mg of PSA, and centrifuged at 10000r / min After 2 minutes, draw the supernatant, pass through a 0.22 μm filter membrane, and wait for detection.

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Abstract

The invention relates to a method for detecting residual amount of quizalofop-p-ethyl in phaseolus calcaratus. The method comprises the steps that firstly, a QuEChERS-pretreatment method which adoptsacetic acid acetonitrile for extraction, adopts sodium chloride and anhydrous magnesium sulfate for drying and adopts N-primary secondary amine for purifying is adopted to pretreat the phaseolus calcaratus, then a high performance liquid chromatograph is applied to be matched with a DAD to detect the quizalofop-p-ethyl, and finally, quantitative analysis and qualitative analysis are conducted. 1,according to the QuEChERS-pretreatment method, the analysis speed is high, and phaseolus calcaratus samples can be treated within 30 min; and 2, only the high performance liquid chromatograph is selected to be matched with the DAD to detect the quizalofop-p-ethyl in the phaseolus calcaratus quickly and efficiently, the purifying effect is good, both the precision and accuracy meet the residue analysis requirements, the defects that a traditional pesticide residue detecting method is complex in operation, long in consumed time, expensive in instrument, poor in purifying effect and the like areavoided, and rapid qualitative and quantitative analysis of the quizalofop-p-ethyl in the phaseolus calcaratus is achieved.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, in particular to a method for detecting quizalofop-ethyl residues in Chixiaodou. Background technique [0002] The application of pesticides is an important factor for the rapid development of agricultural production in the 20th century, but the unreasonable use of pesticides will also bring serious risks to people, and the results of pure toxicology including health toxicology and environmental toxicology assessment and residue assessment are difficult to be comprehensive Objectively speaking, based on the existing toxicological evaluation and residue evaluation results, my country has successively banned a number of highly toxic and high residue pesticides, which has played a certain role in controlling the risk of pesticide application. However, compared with developed countries, my country's pesticide However, there is still a relatively large security risk in the use of it. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14
Inventor 汤华成屈江玲曹冬梅
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY