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Preparation method of aptamer biosensor for t-2 toxin detection in grain or feed

A biosensor, T-2 technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as toxin residues and threats to human health, achieve high-sensitivity detection, improve biocompatibility, and increase sensitivity

Active Publication Date: 2021-05-25
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

In addition, the consumption of contaminated feed by animals will cause toxin residues in animal-derived food, which also indirectly threatens human health

Method used

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  • Preparation method of aptamer biosensor for t-2 toxin detection in grain or feed
  • Preparation method of aptamer biosensor for t-2 toxin detection in grain or feed
  • Preparation method of aptamer biosensor for t-2 toxin detection in grain or feed

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Embodiment 1

[0045] 1) First, add 5mg of molybdenum disulfide into 5mL of ultrapure water, ultrasonically disperse in an ultrasonic instrument for use, then add 100μL of aniline solution into 10mL of sulfuric acid solution with a concentration of 0.5M under continuous stirring, and then change to Stir vigorously, add dropwise 10ml of potassium persulfate with a concentration of 0.15M, and then add 5mL of molybdenum disulfide with a concentration of 1mg / mL to the above solution. After stirring evenly, place in an ice bath at 0°C for 4 hours. Then wash with ultrapure water at 8000rpm / 5min three times to obtain molybdenum disulfide-polyaniline composite, and dry the material in a vacuum oven for use. Take 2 mg of the dried molybdenum disulfide-polyaniline complex and dissolve it in 2 mL of 1% acetic acid, dissolve it evenly by ultrasonication, then add 2 mg of chitosan, oscillate and mix, place it on a stirrer and stir vigorously, add 2 mL of 2% chloroauric acid, Then slowly add 2 mL of sodiu...

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Abstract

The invention relates to a preparation method and application of an aptamer biosensor for detecting T-2 toxin in grain or feed, and belongs to the technical field of electrochemical detection. It is characterized in that: first synthesize gold nanorods (Au NRs) material, then reduce platinum on the gold nanorods to form gold platinum nanorods (Au@PtNRs), through reducing graphene oxide-tetraethylenepentamine ( rGO‑TEPA) to increase the immobilized capacity and improve the signal amplification capability. Then, by combining with single-stranded DNA signal probes, biological signal probes were prepared; finally, T ‑2 toxin detection aptamer sensor, which was successfully applied to the trace detection of T‑2 toxin in grain or feed. The advantage of the present invention is that it breaks through the limitation of small molecular substances of T-2 toxin, and realizes trace detection of T-2 toxin with high sensitivity and high specificity by introducing nucleic acid aptamer. It provides a simple and quick new strategy for the detection of mycotoxins.

Description

Technical field: [0001] The invention relates to a preparation method and application of an aptamer sensor for quantitatively detecting T-2 toxin in grain or feed, and uses the mechanism of specific recognition of T-2 toxin and its aptamer to develop a nucleic acid probe, and Signal probes were prepared by combining with reduced graphene oxide-tetraethylenepentamine and gold-platinum nanorod composites, so as to develop a new type of aptasensor for the detection of T-2 toxin in food or feed, which belongs to electrochemical detection field. Background technique: [0002] T-2 toxin is one of the most toxic trichothecene mycotoxins produced by the metabolism of Fusarium fungi (mainly Cladosporium fusarium) in the mildew process of grains, cereal products and animal feed. It can cause multiple Functional system damage seriously endangers life and health, and is considered by the European Food Safety Authority to be one of the most dangerous pollutants in food. People and anim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 何俊琳于超钟航天
Owner CHONGQING MEDICAL UNIVERSITY
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