Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for creating male sterile lines of plants

A plant and fertility technology, applied in the field of creating plant male sterile lines, can solve the problem that fertility is easily affected by weather conditions, and achieve the effects of speeding up plant breeding, expanding the sources of plant sterile lines, and reducing breeding costs.

Active Publication Date: 2021-12-21
TSINGHUA UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method greatly improves the breeding efficiency, and it is less difficult to breed light-temperature-sensitive male sterile lines, but the fertility of light-sensitive and temperature-sensitive lines is easily affected by weather conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for creating male sterile lines of plants
  • Method for creating male sterile lines of plants
  • Method for creating male sterile lines of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, creation of rice male sterile line

[0059] In this example, Crispr / Cas9 was used to knock out the OsOPR7 gene in rice, and a male sterile line of rice was obtained. The male sterile line could restore its partial fertility after spraying methyl jasmonate. The sequence is sequence 1 in the sequence listing, its CDS sequence is sequence 2 in the sequence listing, and encodes the OsOPR7 protein shown in sequence 3 in the sequence listing. The specific operation steps are as follows:

[0060] 1. Construction of Crispr / Cas9 knockout vector

[0061] The Crispr / Cas9-OsOPR7 vector used to knock out the OsOPR7 gene in rice was constructed, and the selected target sequence was: 5′-GAAGGTTTCCTCATGTCCCT-3′ (position 1207-1226 of sequence 1 in the sequence listing). The steps to construct the Crispr / Cas9-OsOPR7 vector are as follows:

[0062] A. Construction of OsSg-OPR7-T1 plasmid: 1) Design and synthesize the primer sequence of OPR7-T1, each taking an equal amoun...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for creating a male sterile line of a plant. The method for creating a plant male sterile line disclosed by the present invention comprises: reducing the content of the protein shown in sequence 3 in the recipient plant, or inhibiting the activity of the protein in the recipient plant, or knocking out the protein in the recipient plant The gene encoding the protein, or the expression of the gene encoding the protein in the recipient plant is reduced to obtain a plant sterile line. The fertility of the plant sterile line obtained by the creation method of the plant sterile line of the present invention can be recovered by applying jasmonin or its derivatives, and is not limited by the genetic background and avoids the negative effect of the sterile cytoplasm, which is useful for expanding the plant sterile line Sources, simplifying the seed production process, accelerating the speed of plant breeding, and reducing the cost of breeding are all of great significance, and can promote the rapid development of hybrid breeding of different plants.

Description

technical field [0001] The invention relates to a method for creating male sterile lines of plants in the field of biotechnology. Background technique [0002] Hybrid breeding is an important means of creating variation and utilizing heterosis, and is one of the most effective breeding methods. Male sterility is the basis and effective tool of hybrid breeding, and has important scientific and industrial value. Male sterile plants refer to the phenomenon that the male reproductive organs of plants cannot produce normally functioning pollen. At present, male sterile lines used for plant breeding are often found from natural populations, with limited species, long breeding cycle, low efficiency and high price. [0003] The development of rice hybrid breeding is the most mature. In the cultivation of three-line hybrid rice, in addition to the sterile line, restorer lines and maintainer lines are also required. Its advantage is that the fertility of the sterile line is stable,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00A01H6/20A01H6/46
CPCC07K14/415C12N15/8213C12N15/8289
Inventor 谢道昕闫建斌鄢纯张晓林怡荣
Owner TSINGHUA UNIV