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Culture method of scenedesmus obliquus and application of scenedesmus obliquus to purification of kitchen waste water and generation of oil

A cultivation method and kitchen wastewater technology are applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., which can solve problems such as consumption, large fresh water resources, and increased cultivation costs, and achieve low cost, Reduce the cost of cultivation and realize the effect of large-scale cultivation

Pending Publication Date: 2019-08-20
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

At present, artificial culture medium is mostly used for microalgae cultivation, which will consume a lot of fresh water resources. In addition, adding a large amount of inorganic salts and organic matter such as nitrogen and phosphorus increases the cost of cultivation.

Method used

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  • Culture method of scenedesmus obliquus and application of scenedesmus obliquus to purification of kitchen waste water and generation of oil
  • Culture method of scenedesmus obliquus and application of scenedesmus obliquus to purification of kitchen waste water and generation of oil

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Embodiment 1

[0025] (1) Kitchen waste water was taken from the waste liquid tank of the canteen of Beijing Forestry University. Pre-treat the wastewater, first filter out large pieces of solid waste, and then let it stand to remove the upper layer of grease.

[0026] (2) Pretreat the pretreatment solution: use 3mol / L HCl to adjust the pH of the wastewater to 5, add 500U of acid protease, 10U of α-amylase, 100U of glucoamylase and 10U of cellulose to each milliliter of pretreatment solution The enzyme is placed in a constant temperature shaker at 50° C. for 2 hours to obtain a kitchen wastewater treatment liquid.

[0027] (3) Dilute the kitchen waste water treatment solution with clean water, adjust the ammonia nitrogen content to 100 mg / L, sterilize at 121° C. for 30 minutes, and use it as a culture solution for Scenedesmus obliquus. Inoculate Scenedesmus obliquus in the logarithmic growth phase into the kitchen wastewater treatment solution at a density of 5×10 5 photons / mL, the ratio o...

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Abstract

The invention provides a culture method of scenedesmus obliquus, and belongs to the technical field of microalgae biological treatment. The culture method comprises the following steps of inoculatingkitchen waste water treatment liquid with scenedesmus obliquus, performing culturing at 20-30 DEG C for 7-13d, and performing separating to obtain frond. According to the method, the kitchen waste water treatment liquid is used as a culture medium of the scenedesmus obliquus, and the scenedesmus obliquus can well grow in the kitchen waste water treatment liquid without additional addition of nutritive salt, so that the culturing cost is reduced. The scenedesmus obliquus can continuously transfer pollutants in the kitchen waste water for proliferation and accumulation of cell fat, besides, thekitchen waste water is purified to protect environment, realization of low-cost scaleable culture of the microalgae is facilitated, and industrial development of the microalgae biodiesel can be promoted.

Description

technical field [0001] The invention belongs to the technical field of microalgae biological treatment, and in particular relates to a cultivation method of Scenedesmus obliquus and its application in purifying kitchen waste water to produce oil. Background technique [0002] With the increasingly serious problems of fossil energy and environmental degradation, clean and renewable energy has become the goal and trend of energy development in various countries. As a renewable energy source, biofuel has been rapidly developed and widely used due to its high efficiency and low pollution. Microalgae has become an excellent raw material for biodiesel production due to its high photosynthetic efficiency, no occupation of agricultural land, ability to fix carbon dioxide, and high lipid content. The use of microalgae to produce biodiesel requires large-scale cultivation to provide sufficient raw materials. At present, artificial medium is mostly used for microalgae cultivation, wh...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F3/32C12R1/89
CPCC02F3/322C12N1/12
Inventor 洪喻李亚丽刘晓亚
Owner BEIJING FORESTRY UNIVERSITY
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