Application of composition to the preparation of medicament for treating abnormal proliferation of collagen after peritoneal damage
A collagen and composition technology, applied in the field of umbilical cord mesenchymal stem cells or umbilical cord mesenchymal stem cell exosomes, can solve the problems of inability to treat peritoneum and reduce the rate of deterioration of peritoneal fibrosis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0094] Example 1. It was shown by Macroscopic that transplantation of human umbilical cord mesenchymal stem cells can treat peritoneal adhesions and abdominal cocoons in mice with peritoneal fibrosis
[0095] Fixed without perfusion ( figure 2 A-E), after fixation with perfusion ( figure 2 The abdominal wall of each group of rats in a-e) was cut open, and the appearance was observed. The results showed that the peritoneum in the abdominal cavity of the normal group was clear and tightly attached to the viscera, so the surface of the liver and small intestine appeared smooth ( figure 2 A and a). Rats in the PD / MGO group were treated with PD / MGO for three weeks, the peritoneum on the surface of the viscera was clearly visible, the edge of the liver became thick and swollen, and the small intestine became hypertrophic due to food accumulation ( figure 2b and b). Rats in the PD / MGO+solvent group were treated with PD / MGO for three weeks, and only given normal saline the nex...
Embodiment 2
[0096] Example 2. Transplantation of human umbilical cord mesenchymal stem cells can reduce the thickening of parietal peritoneum and visceral peritoneum in mice with peritoneal fibrosis
[0097] The parietal peritoneum ( image 3 A-E), and visceral peritoneum ( image 3 G-K), H&E staining was performed to observe the change of peritoneal thickness. The results showed that in the normal group, no matter the parietal peritoneum on the surface of the rectus abdominis muscle or the visceral peritoneum on the surface of the liver, there was only a thin layer ( image 3 A and G). In PD / MGO group, treated with PD / MGO for three weeks, the peritoneal thickness was significantly thickened ( image 3 B and H). The thickness of the visceral peritoneum and parietal peritoneum in the PD / MGO+vehicle group was significantly higher than that in the PD / MGO group, and they also adhered to each other ( image 3 C and I).
[0098] In the PD / MGO+HUMSCs(LD) group, the peritoneal thickness of ...
Embodiment 3
[0099] Example 3: Transplantation of human umbilical cord mesenchymal stem cells can reduce collagen in the parietal peritoneum and visceral peritoneum of mice with peritoneal fibrosis
[0100] The parietal peritoneum ( Figure 4 A-E), and visceral peritoneum ( Figure 4 G-K), Sirius red staining was performed, and collagen was marked to observe the changes of intraperitoneal collagen. The results showed that there was a small amount of collagen in the parietal peritoneum or visceral peritoneum of the normal group ( Figure 4 A and G). In the PD / MGO group, treated with PD / MGO for three weeks, the thickened intraperitoneum had massive accumulation of collagen ( Figure 4 B and H). The parietal peritoneum and visceral peritoneum in the PD / MGO+solvent group not only became thicker, but also the collagen content in the thickened peritoneum and the collagen between the muscle tissue increased significantly ( Figure 4 C and I). In the PD / MGO+HUMSCs(LD) group, the collagen in ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More