Detection method of exogenous gene and kit

Abstract

The invention discloses a detection method of an exogenous gene and a kit, and belongs to the field of biological detection. The detection method comprises the following steps: determining the exogenous gene needed to be detected in a sample to be detected and an endogenous standard gene in the sample to be detected; preparing a hybridization probe according to an artificial sequence and a bordersequence of the exogenous gene or a sequence of the endogenous standard gene; extracting and purifying genome DNA of the sample to be detected and genome DNA of a quality control sample and performingfragmentation respectively; respectively connecting adapter sequences to DNA fragments of the sample to be detected and DNA fragments of the quality control sample; performing detection on the exogenous gene in the sample to be detected by using template fragments and characteristic fragments of the exogenous gene, and judging whether the exogenous gene exists in the sample to be detected. According to the method, a false positive result caused by microbiological infection and aerosol pollution can be avoided, and the problem of inaccurate quantification caused by factors of PCR amplificationefficiency and the like can also be avoided.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection method and kit for exogenous genes. Background technique [0002] The commercial development of genetically modified crops appeared in 1996. With the development of biotechnology, the types of genetically modified plant products are increasing year by year. According to the report of the International Service for the Acquisition of Agri-biotech Applications (ISAAA), as of 2013, 27 types of genetically modified crops and 336 varieties of genetically modified crops have been developed in the world. In the past 27 years, the global planting area of ​​genetically modified crops has reached 175 million hectares. In China, as of 2015, 37 genetically modified crops have been approved for import and used as raw materials for processing. In many countries, including China, the government and the public are concerned about the food safety or environmental safety of genetic...

AI Technical Summary

Problems solved by technology

Even so, false positives in GMO testing due to pollution often occur, and even qualified laboratories often affect test results due to aerosol pollution

Existing GMO detection methods may be infected by microorganisms, resulting in false positive test results